We narrowed to 20,317 results for: Kars

Showing: 4641 - 4660 of 20317 results

Results per page:

Sort results by:

pET28a-MH6-EsCas13d

Plasmid

#108303

Purpose

Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

EsCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-Dm4EHP-W114A_AH

Plasmid

#148909

Purpose

Insect Expression of Dm4EHP-W114A

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

Dm4EHP-W114A (4EHP Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pC003 - LshC2C2 locus into pACYC184 for spacer cloning

Plasmid

#79152

Purpose

LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Mutation

*

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDropIn-T2A-ZpGal4DBD

Plasmid

#199199

Purpose

Insert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-ZpGal4DBD

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDropIn-T2A-p65Zp

Plasmid

#199200

Purpose

Insert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-p65Zp

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pY010 (pcDNA3.1-hAsCpf1)

Plasmid

#69982

Purpose

Expresses humanized AsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hAsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_iNEUROD1_P2A_GFP_Puro

Plasmid

#168803

Purpose

PiggyBac transposon construct with doxycycline inducible NEUROD1 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

NEUROD1 (NEUROD1 Synthetic, Human)

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0336 pAAV EFS-HvsCas7-11 for dual vector

Plasmid

#172515

Purpose

Encodes HvsCas7-11 mammalian expression casette in AAV backbone

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV EFS-HvsCas7-11 for dual vector

Use

AAV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SIN40C.SFFV.Luciferase.IRES.GFP

Plasmid

#169308

Purpose

Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-EGFP

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP VAMP3

Plasmid

#42310

Depositor

Thierry Galli

Article

Galli et al Mol Biol Cell. 1998 Jun;9(6):1437-48.

Insert

VAMP 3 (VAMP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-sgRNA expression vector

Plasmid

#51132

Purpose

For in vitro transcription of sgRNA from the T7 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTXB1.Tat(48-57)-Cre recombinase

Plasmid

#165042

Purpose

Expression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

Cre recombinase

Tags

Mxe Intein-Chitin binding domain and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

Plasmid

#61594

Purpose

A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D10A, N580A

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

Plasmid

#32702

Purpose

Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Insert

eGFP

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available Since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
shh10

Plasmid

#64867

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

Insert

shh10 cap

Use

AAV

Mutation

I319V, N451D, D532N

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIG3 (pCMV-IRES-GFP version 3)

Plasmid

#78264

Purpose

Lentiviral empty cloning vector containing IRES and GFP

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV, lentiviral LTR

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR v2-sgLKB1 clone2

Plasmid

#162126

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-ER-TurboID

Plasmid

#200965

Purpose

3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

ER-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG_hPPP1R15B_4-713_mCherry_UK1298

Plasmid

#80707

Purpose

mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)

Depositor

David Ron

Article

Chen et al Elife. 2015 Mar 16;4. doi: 10.7554/eLife

Insert

human PPP1R15B (PPP1R15B Human)

Tags

mCherry

Expression

Mammalian

Mutation

CDS 4-713 no stop codon

Promoter

CMV

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge