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pSMP-Bmi1_3

Plasmid

#36354

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-G9A_2

Plasmid

#36332

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pdestMB14

Plasmid

#26415

Depositor

Marc Vidal

Article

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2

Expression

Bacterial and Worm

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

Plasmid

#216155

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with cryptic splice site and C-terminal FLAG tag

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CCreV

Plasmid

#140132

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CCreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NCreV

Plasmid

#140131

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

NCreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFSynW Substance P-pHluorin IRES mRuby3

Plasmid

#195699

Purpose

Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Tac1 (Tac1 Mouse)

Use

Lentiviral

Tags

pHluorin

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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TLS 1: hTLS.pBSKS(+)

Plasmid

#21827

Depositor

David Ron

Article

Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Insert

TLS (FUS Human)

Expression

Bacterial

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2+ EGFP-3NLS

Plasmid

#165400

Purpose

Expresses nuclear localized EGFP

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

Nuclear Localization Signal

Use

Avian, xenopus, zebrafish

Tags

EGFP

Expression

Mammalian

Promoter

SP6

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEB1-mGFPmut3

Plasmid

#103981

Purpose

Plasmid encoding mGFPmut3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65G, S72A, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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sZF15

Plasmid

#43846

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF15

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
sZF1

Plasmid

#43832

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF1

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pFA6a-GFP(S65T)-TRP1

Plasmid

#41597

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP (S65T) and yeast ADH1 terminator

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-13Myc-TRP1

Plasmid

#41601

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

13Myc and yeast ADH1 terminator

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pAdEasy-EGFP-CLASP2 340-1362

Plasmid

#24522

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1362) (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant that retains MT lattice bi…

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pAC3-ISH

Plasmid

#15854

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Adenylate Cyclase 3 (Adcy3 Mouse)

Expression

Bacterial

Mutation

contains residues 787-1021

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
GST-FOXO3a 1-506aa

Plasmid

#10826

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

GST-FOXO3a 1-506aa (FOXO3 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb-2xKS

Plasmid

#238236

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-2xKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-2xKS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVAX1-cGAS

Plasmid

#196624

Purpose

Express mouse cGAS protein in mammalian cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

cGAS (Cgas Mouse)

Expression

Mammalian

Mutation

WT

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-off Full length Tau (0N4R, P301L/V337M)

Plasmid

#188573

Purpose

Dox-repressible expression of the 0N4R Tau isoform

Depositor

Franz-Ulrich Hartl

Article

Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

Insert

0N4R Tau (MAPT Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P301L and V337M

Promoter

pTight TRE

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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