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We narrowed to 60,007 results for: tra

Showing: 4641 - 4660 of 60007 results
  1. pAAV uN2CpolyGly op GFP (NIID)

    Plasmid
    #224356
    Purpose
    Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)
    Insert
    uN2C with a GGC repeat expansion (100x)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Codon-optimized for expression in human, so no pu…
    Promoter
    CMV + chimeric intron (CAG)
    Available Since
    Feb. 5, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ

    Plasmid
    #178320
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.
    Depositor
    Inserts
    HRE RedFirefly reporter
    P53 FLuc reporter
    AP-1 Renilla reporter
    FOXO NLuc Reporter
    SMAD GrRenilla reporter
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid
    #179905
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Insert
    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…
    Promoter
    CAG
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

    Plasmid
    #172597
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.
    Depositor
    Insert
    myc-PDGFRA-K627R (PDGFRA Synthetic, Human)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-K627R
    Promoter
    CMV
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pDCAF3-IS5

    Plasmid
    #65220
    Purpose
    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.
    Depositor
    Inserts
    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Changed start codon from gtg to atg and Changed s…
    Promoter
    BBa_J23100
    Available Since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid
    #186427
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)
    Depositor
    Inserts
    KanR
    BastaR
    Rb7
    eGFP
    Use
    Synthetic Biology; Binary vector for escherichia …
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter

    Plasmid
    #172596
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.
    Depositor
    Insert
    myc-PDGFRA-R914W (PDGFRA Synthetic, Human)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-R914W
    Promoter
    CMV
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. Nav1.5_1-2linker411-717_PPSYtoAASA1960-1996

    Plasmid
    #187732
    Purpose
    Express a chimera of the Nav1.5 linker between the first and second transmembrane domain with the motif surrounding PPSY; residues 1974-1977 PPSY mutated to AASA
    Depositor
    Insert
    Nav1.5 1,2linker residues 411-717 and Nav1.5 residues 1960-1996 PPSY mutated to AASA (SCN5A Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    deletion of residues 1-410 and 718-1959 and 1997-…
    Available Since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter

    Plasmid
    #172598
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.
    Depositor
    Insert
    myc-PDGFRA-D842V (PDGFRA Synthetic, Human)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-D842V
    Promoter
    CMV
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter

    Plasmid
    #172599
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.
    Depositor
    Insert
    myc-PDGFRA-D842V+R914W (PDGFRA Synthetic, Human)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-D842V+R914W
    Promoter
    CMV
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter

    Plasmid
    #172600
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.
    Depositor
    Insert
    myc-PDGFRA-K627R+R914W (PDGFRA Synthetic, Human)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-K627R+R914W
    Promoter
    CMV
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pT2iU6Apowriter2N(#355)

    Plasmid
    #184106
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis + in vitro trans…
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 4641 - 4660 of 60007 results