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We narrowed to 63,812 results for: cloning

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Showing: 4661 - 4680 of 63812 results

  6. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

    Plasmid
    #114073
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548R and N282A
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114073-plasmid-map-sequence-id-220529

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  9. pEBTet_NADP-Snifit-mito

    Plasmid
    #113919
    Purpose
    Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells
    Depositor
    Kai Johnsson
    Publication
    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NADP-Snifit
    Expression
    Mammalian
    Promoter
    CMV TetO2
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    113919-plasmid-map-sequence-id-215103

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  14. pCS6-YFP-SMASh(TI)

    Plasmid
    #111501
    Purpose
    Expresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].
    Depositor
    Michael Lin
    Publication
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP-SMASh(TI)
    Tags
    HA epitope tag and SMASh tag
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries F43L, Q80K, S122R, a…
    Promoter
    CMV
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111501-plasmid-map-sequence-id-214268

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  17. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid
    #106965
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106965-plasmid-map-sequence-id-207982

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  19. pDHt/sk-PE

    Plasmid
    #92126
    Purpose
    Expression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reesei
    Depositor
    Zhihua Zhou
    Publication
    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-eGFP
    Use
    CRISPR; Trichoderma reesei expression
    Tags
    EGFP
    Promoter
    Ppdc
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92126-plasmid-map-sequence-id-195058

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  20. pBADTrfp

    Plasmid
    #99382
    Purpose
    This promoter system provided the highest RFP expression upon induction
    Depositor
    Nathan Hillson
    Insert
    RFP
    Expression
    Bacterial
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99382-plasmid-map-sequence-id-192484

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Showing: 4661 - 4680 of 63812 results