We narrowed to 24,891 results for: promoter

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pPANI2A2E2-Crimson

Plasmid

#193758

Purpose

Expresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

E2-Crimson

Use

Synthetic Biology

Expression

Bacterial

Promoter

PANI2 synthetic promoter carrying binding sites f…

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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htpG2-GFP

Plasmid

#109388

Purpose

GFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

phtpG2-GFP

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pZmUbi-5'UTR

Plasmid

#154057

Purpose

Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

Promoter and 5' UTR Ubiquitin (Zea mays)

Use

Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHes1-GFP (CC#109)

Plasmid

#15133

Depositor

Connie Cepko

Article

Furukawa et al Neuron. 2000 May . 26(2):383-94.

Insert

Hes1 (Hes1 Rat)

Use

Retroviral

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 C506S

Plasmid

#78584

Purpose

Expression flag-USP19 C506S in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 C506S (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutate 506 cysteine residue to serine

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pT7-SpCas9_sgRNA-site1 (RTW443)

Plasmid

#160136

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX-p53 R175H

Plasmid

#39480

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

TP53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

P72R and R175H

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only
NK73

Plasmid

#176609

Purpose

To test bead engulfment of macrophages.

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

SNAP-CD86-FCERG (CD86 Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Mutation

n/a

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_212

Plasmid

#133457

Purpose

U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-nucGFP11-3xControlgRNA

Plasmid

#224568

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

GFP11-H2B

Use

CRISPR

Tags

Histone H2B (nuclear localization)

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-p53

Plasmid

#39479

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

TP53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

P72R

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only
pXPR_213

Plasmid

#133456

Purpose

H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

Control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-crScaffold_SV40-BFP

Plasmid

#224860

Purpose

crRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 beta-catenin Y654E

Plasmid

#16073

Depositor

Erin Schuman

Article

Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

Insert

beta-catenin (CTNNB1 Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

Y654E, *(see below)

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I delta isoform

Plasmid

#22188

Depositor

Ananda Roy

Article

Cheriyath et al Mol Cell Biol. 1998 Aug . 18(8):4444-54.

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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MBD1v1 pcDNA3.1

Plasmid

#78140

Purpose

Mammalian expression of MBD1v1

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MBD1 (MBD1 Human)

Expression

Mammalian

Mutation

v1 isoform

Promoter

CMV

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
USP19 sgRNA2

Plasmid

#78586

Purpose

delete USP19 gene in human cell

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 sgRNA2 (USP19 Human)

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pLPP3

Plasmid

#209965

Purpose

Contains Level 0 Part: constitutive Promoter (P25) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P25)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPP7

Plasmid

#209969

Purpose

Contains Level 0 Part: autoinducible Promoter (PfabZ) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_fabZ)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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