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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,508 results for: crispr

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Showing: 4681 - 4700 of 25508 results

  4. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid
    #185911
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185911-plasmid-map-sequence-id-367364

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  5. pUS2-SH231

    Plasmid
    #115150
    Purpose
    Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4
    Depositor
    Raymond Monnat, Michael Phelps
    Publication
    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dual SH231 gRNAs
    Use
    CRISPR
    Tags
    3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    U6, CBh
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115150-plasmid-map-sequence-id-224718

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  8. CROPSeq-BFP

    Plasmid
    #196718
    Purpose
    CROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFP
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196718-plasmid-map-sequence-id-388068

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  9. SID4x-dCas9-KRAB

    Plasmid
    #106399
    Purpose
    Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain
    Depositor
    Jason Gertz
    Publication
    Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SID4x (MXD1 Human)
    KRAB (ZNF10 Human)
    Tags
    FLAG, HA, and NLS
    Expression
    Mammalian
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106399-plasmid-map-sequence-id-207594

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  15. ePPEplus

    Plasmid
    #205241
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Publication
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205241-plasmid-map-sequence-id-408831

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Showing: 4681 - 4700 of 25508 results