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Ac5-STABLE2-puro

Plasmid

#208032

Purpose

Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo

Plasmid

#153422

Purpose

MSCV with IRES-Neomycin resistant gene

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Cox8x2-CeNL_pcDNA3

Plasmid

#89513

Purpose

Cyan color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

CoxVIII X2

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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tubulin-OeNL_pcDNA3

Plasmid

#89523

Purpose

Orange color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

b-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-csGBP

Plasmid

#108891

Purpose

Multisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

csGBP

Use

Multisite gateway

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pME-miniTurbo

Plasmid

#166567

Purpose

Multisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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CeNL_pRSETb

Plasmid

#89510

Purpose

Cyan color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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Cox8x2-OeNL_pcDNA3

Plasmid

#89526

Purpose

Orange color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

CoxVIII X2

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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VV073: 1xCox8 - GCaMP5G in fubi

Plasmid

#58509

Purpose

Expresses the calcium sensor GCaMP5G in the mitochondria

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - GCaMP5G

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pDGE503

Plasmid

#153276

Purpose

Intermediate sgRNA array cloning vector position 1

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE504

Plasmid

#153277

Purpose

Intermediate sgRNA array cloning vector position 2

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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HIV-1 integrase F185K/C280S in pET-15b

Plasmid

#61669

Purpose

HIV-1 integrase F185K/C280S in pET-15b

Depositor

Robert Craigie

Article

Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

Insert

HIV-1 integrase F185K/C280S

Use

Synthetic Biology

Tags

His

Promoter

T7 promotor

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pME-TurboID-NS

Plasmid

#166566

Purpose

Multisite Gateway middle entry clone encoding TurboID. Parton lab clone JRP

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSPORT-RREB1

Plasmid

#41145

Depositor

Joshua Mendell

Article

Kent et al Oncogene. 2012 Jul 2. doi: 10.1038/onc.2

Insert

RREB-1 (RREB1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

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ReNL-H2B_pcDNA3

Plasmid

#89534

Purpose

Red color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

histone 2B

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-beta2-microglobulin

Plasmid

#15883

Depositor

Peter Mombaerts

Article

Ishii et al Curr Biol. 2003 Mar 4. 13(5):394-400.

Insert

beta2-microglobulin (B2m Mouse)

Available Since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO_HaloTag9_LamB1

Plasmid

#175538

Purpose

Expression of HaloTag9 at the nuclear lamina of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9-LamB1

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pVITRO1-iRFP702-LaminB1

Plasmid

#184456

Purpose

Nuclear membrane-targeted iRFP702 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

iRFP702-LaminB1

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-BFP-CAAX

Plasmid

#184459

Purpose

Plasma membrane-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-CAAX

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9FL-P2A-turboGFP

Plasmid

#80941

Purpose

Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9FL

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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