Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,927 results for: MLS

Clear keywords
Showing: 4721 - 4740 of 9927 results

  2. K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

    Plasmid
    #184563
    Purpose
    CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.
    Depositor
    Kacper Rogala
    Article
    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:
    Insert
    C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    All lysines were mutated to arginines. Codon-opti…
    Promoter
    CMV
    Available Since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    184563-plasmid-map-sequence-id-376975

    Enlarge

  6. Donor-CD55-NeoR

    Plasmid
    #153551
    Purpose
    Used as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Mutation
    a 1-bp mutation in exon 4
    Promoter
    No promoter
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    153551-plasmid-map-sequence-id-326384

    Enlarge

  7. Donor-PIGA-NeoR

    Plasmid
    #153549
    Purpose
    Used as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Mutation
    a 3-bp mutation in exon 6
    Promoter
    No promoter
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    153549-plasmid-map-sequence-id-319273

    Enlarge

  8. pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

    Plasmid
    #123336
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Cerulean3-cpCerulean3-FLARE-AKAR
    Tags
    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123336-plasmid-map-sequence-id-307044

    Enlarge

  12. LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR (DRR728)

    Plasmid
    #248232
    Purpose
    Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attP target sites
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586
    Insert
    LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    None
    Promoter
    NA
    Available Since
    April 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    248232-plasmid-map-sequence-id-506813

    Enlarge

  13. pCMV-bpNLS-Stab-exin21-Bxb1(A315R)-Stab (CJT605)

    Plasmid
    #248211
    Purpose
    bpNLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586
    Insert
    bpNLS-Stab-exin21-Bxb1(A315R)-Stab
    Use
    CRISPR; In vitro transcription
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Bxb1(A315R)
    Promoter
    CMV and T7
    Available Since
    April 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248211-plasmid-map-sequence-id-506607

    Enlarge

Showing: 4721 - 4740 of 9927 results