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Showing: 1151 - 1200 of 2660 results

  1. enIscB-T5E

    Plasmid
    #205411
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E85R+H369R+S387R+S457R
    Promoter
    CAG, hU6, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205411-plasmid-map-sequence-id-416557

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  5. PB-iRFP-STOP-ReNL

    Plasmid
    #113965
    Purpose
    Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)
    Depositor
    Jordan Green
    Article
    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:
    Inserts
    iRFP670
    ReNL
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV - SV40-PolyA - SV40-PolyA and EF1A
    Available since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    113965-plasmid-map-sequence-id-223037

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  6. AdMKP-3 S159AS197A

    Plasmid
    #131523
    Purpose
    Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells
    Depositor
    Haiyan Xu
    Article
    Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2
    Insert
    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    Use
    Adenoviral
    Tags
    No
    Expression
    Mutation
    changed Serine 159 to Alanine and Serine 197 to A…
    Promoter
    CMV
    Available since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    131523-plasmid-map-sequence-id-256915

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  8. pNHA_SBV_Gc

    Plasmid
    #208933
    Purpose
    Expresses N-terminally HA-tagged Schmallenberg orthobunyavirus Gc protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Gc
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 1682 bp from 5' end, deleted 378 bp …
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208933-plasmid-map-sequence-id-419021

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  14. ShuttleMCP

    Plasmid
    #50959
    Purpose
    A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoter
    Depositor
    James Bamburg
    Article
    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    uses portion of the mouse cofilin gene as a mouse…
    Available since
    Feb. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    50959-plasmid-map-sequence-id-76720

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  29. pZFN-AAVS1-KKR

    Plasmid
    #159298
    Purpose
    ZFN (right) targeting the AAVS1 locus with obligate heterodimer FokI
    Depositor
    Kosuke Yusa
    Insert
    ZFN targeting the AAVS1 locus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    159298-plasmid-map-sequence-id-311594

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  30. pLenti-TRE-GFP-Blast

    Plasmid
    #135673
    Purpose
    Dox-inducible expression (TRE promoter) of GFP cDNA (control for other cDNAs), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135673-plasmid-map-sequence-id-327577

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  36. pNHA_SBV_NSs

    Plasmid
    #208932
    Purpose
    Expresses N-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 50 bp from 5' end (including start c…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208932-plasmid-map-sequence-id-419004

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  37. pNHA_OROV_Gc

    Plasmid
    #208936
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus Gc protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Gc
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 1579 bp from 5' end, deleted 277 bp …
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208936-plasmid-map-sequence-id-418962

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  38. pCHA_OROV_NSs

    Plasmid
    #208939
    Purpose
    Expresses C-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 66 bp from 5' end and deleted 415 b…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208939-plasmid-map-sequence-id-419030

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  39. pNHA_OROV_NSm

    Plasmid
    #208935
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSm
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 949 bp from 5' end (Gn), deleted 29…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208935-plasmid-map-sequence-id-419020

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  41. pNX24-RFP-MS2SL

    Plasmid
    #227819
    Purpose
    Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene
    Depositor
    Jeffrey Gerst
    Article
    Haimovich et al bioRxiv 2024.11.06.622258
    Inserts
    3xHA-RFP-24xMS2SL
    Neo resistance
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and SV40
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    227819-plasmid-map-sequence-id-469648

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  42. pCHA_SBV_NSs

    Plasmid
    #208938
    Purpose
    Expresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 47 bp at 5' end and 510 bp from 3&#x…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208938-plasmid-map-sequence-id-419026

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  43. pCDH-EF1-copGFP-T2A-Puro

    Plasmid
    #72263
    Purpose
    Express copGFP and puromycin resistance gene under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72263-plasmid-map-sequence-id-134557

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  44. pCDH-EF1-DIO-copGFP

    Plasmid
    #72253
    Purpose
    Express copGFP under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    copGFP
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72253-plasmid-map-sequence-id-131724

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  46. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  47. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  48. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  49. pCDH-EF1-DIO-tdTomato

    Plasmid
    #72254
    Purpose
    Express tdTomato under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    72254-plasmid-map-sequence-id-134462

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  50. pCDH-EF1-Nluc

    Plasmid
    #73024
    Purpose
    Express Nluc reporter under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73024-plasmid-map-sequence-id-133955

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Showing: 1151 - 1200 of 2660 results