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Plasmid#104819PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-2). Also expresses Cas9 from Gmubi promoter.DepositorInsertGlyma.04g057400
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC29
Plasmid#104803PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr4g094545 (Hen1). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr4g094545
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCS mCherry
Plasmid#91769PurposeBacterial expression of a fluorescent protein, monomeric Cherry (mCherry)DepositorInsertmonomeric Cherry
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationSee Depositor CommentsPromoterT7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPa-FRB-pre mGRASP
Plasmid#73145PurposeLarge fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.DepositorInsertsHRPa-FRB-pre mGRASP
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Synapsin sHRPa-NRX in FSW lentiviral vector
Plasmid#73147PurposeLarge fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.DepositorInsertsHRPa-NRX
UseLentiviralTags2x HA tag and Flag tagExpressionMammalianMutationPromoterSynapsinAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-Golgin-84-TEV #709#243
Plasmid#42108DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsGFPExpressionMammalianMutationinserted ENLYFQG b/w 300 and 301PromoterCMVAvailable sinceFeb. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-GFP
Plasmid#175363PurposeZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cellsDepositorInsertsgGFP
UseTagsExpressionInsectMutationF64L, S65C, and I167TPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
hP2X3-cALFA
Plasmid#160499PurposeFor the expression of human P2X3 with ALFA tag at C-terminus in mammalian cellsDepositorInserthuman P2X3 (P2RX3 Synthetic, Human)
UseTagsHRV3C protease cleavage site-ALFA tag-twin Strep …ExpressionMammalianMutationΔN5ΔC33-T13P/S15V/V16IPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-mEGFP
Plasmid#191847PurposeExpresses the monomeric form of EGFPDepositorInsertmEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSC43
Plasmid#104817PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from Gmubi promoter from rolD promoterDepositorInsertGlyma.04g057400
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
AtS/MAR10 insulator sequence (GB3458)
Plasmid#160638PurposepEGB3a1 AtS/MAR10DepositorInsertAtS/MAR10 insulator sequence
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC50
Plasmid#104824PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.DepositorInsertGlyma.08g081600, Glyma.05g126600
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
FB399
Plasmid#203635PurposeModule for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).DepositorInsertFB367+FB396
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB400
Plasmid#203636PurposeModule for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).DepositorInsertFB367+FB397
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC51
Plasmid#104825PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoterDepositorInsertGlyma.12g075700, Glyma.11g145900
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ADAR2DD_E488Q
Plasmid#205598Purposeexpresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cellsDepositorInsertADAR2 deaminase domain (ADARB1 Human)
UseTagsExpressionMammalianMutationE488Q hyperactive mutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ADAR2DD_E488Q_Ago2
Plasmid#205599Purposeexpresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cellsDepositorInserthAgo2 (AGO2 Human)
UseTagsADAR2DD_E488Q (hyperactive mutant)ExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPb-NLG
Plasmid#73144PurposeSmall fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.DepositorInsertsHRPb-NLG (Nlgn1 Synthetic, Mouse)
UseTagsV5 tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_T6B -mCherry
Plasmid#205600Purposeexpresses human T6B peptide fused with mChery in mammalian cellsDepositorInsertT6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1
Plasmid#79914PurposepiggyBac transposon vector, Zeocin-2A-sHRPbNLG1DepositorInsertZeocin-t2A-splitHRP-aNLG1 (Nlgn1 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only