We narrowed to 14,164 results for: nts

1. pNTI828

   Plasmid

   #216636

   Purpose

   cpTurboID-ERM (V5) ER membrane-localized cpTurboID, mammalian expression and lentivirus production

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   cpTurboID-ERM
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNTI935

   Plasmid

   #216638

   Purpose

   cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian expression and lentivirus production

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   cpTurboID-PM
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO APEX2-eIF4E1

   Plasmid

   #129644

   Purpose

   Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   EIF4E1 (EIF4E Human)
   

   Use

   Tags

   APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSP1594

   Plasmid

   #65775

   Purpose

   Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPRai

   Plasmid

   #189938

   Purpose

   Expresses CRISPRai cassette under EF1a promoter

   Depositor

   Yu-Chen Hu

   Article

   Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

   Insert

   CRISPRai cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer-rat EF1 alpha

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57.1-ZNF746

   Plasmid

   #207825

   Purpose

   Dox-inducible expression of ZNF746

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   ZNF746 (ZNF746 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNTI826

   Plasmid

   #216634

   Purpose

   cpTurboID (V5) for AAV production

   Depositor

   Nicholas Ingolia

   Article

   Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

   Insert

   cpTurboID
   

   Use

   AAV

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP2283

   Plasmid

   #70702

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. JEH127

   Plasmid

   #179299

   Purpose

   Expresses dCas9 fused to VPR

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

   Insert

   dCas9-VPR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   CAG

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSP2266

    Plasmid

    #70705

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. BPK1160

    Plasmid

    #179298

    Purpose

    Expresses dCas9 fused to P65

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-p65
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBW1997_pExpr-hU6-CRISPR-DECODER

    Plasmid

    #87549

    Purpose

    Expresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    gRNAs targeting NGN2, MIAT, ACTC1, TTN
    

    Use

    CRISPR, Cre/Lox, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MCV-HF LTS220A/S239A

    Plasmid

    #112191

    Purpose

    Merkel cell polyomavirus molecular clone

    Depositor

    Patrick Moore

    Article

    Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

    Insert

    MCV-HF LTS220A/S239A
    

    Use

    Tags

    Expression

    Mutation

    T to G mutations at 3313 (nt) and 3370 (nt) of fu…

    Promoter

    None from vector. Insert contains native promoter…

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYO539

    Plasmid

    #235752

    Purpose

    Protein expression of ScVPS30 and ScVPS38

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Inserts

    VPS30 (VPS30 Budding Yeast)
    VPS38 (VPS38 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL-TDH3

    Available since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYO734

    Plasmid

    #235753

    Purpose

    Protein expression of ScVPS30 and ZZ-ScVPS38

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Inserts

    VPS30 (VPS30 Budding Yeast)
    VPS38 (VPS38 Budding Yeast)
    

    Use

    Tags

    ZZ-3xTEV

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYO934

    Plasmid

    #235766

    Purpose

    Expression of ScVPS34 delta CBR1 L31-D55-3xFLAG undeer its own promoter

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VPS34 delta CBR1
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    aa 31-55 deleted

    Promoter

    ScVPS34 promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYO760

    Plasmid

    #235759

    Purpose

    Expression of ScVPS38 delta C2 (1-154)-x3FLAG under its own promoter

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VPS38 delta C2
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    aa 1-154 deleted

    Promoter

    ScVPS38 promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYO761

    Plasmid

    #235760

    Purpose

    Expression of ScVPS38 delta BARA2 (320-440)-x3FLAG under its own promoter

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VSP38 delta BARA2
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    aa 320-440 deleted

    Promoter

    ScVPS38 promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYO765

    Plasmid

    #235762

    Purpose

    Expression of ScVPS38 delta C2 (1-154) -EGFP under its own promoter

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VPS38
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    aa 1-154 deleted

    Promoter

    ScVPS38 promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYO766

    Plasmid

    #235763

    Purpose

    Expression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoter

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    VSP38 delta BARA2
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    aa 320-440 deleted

    Promoter

    ScVPS38 promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge