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Plasmid#79013PurposeFor mammalian expression of a shortened, constitutively active version of Tfeb.DepositorInsertTfeb (TFEB Human)
UseTagsExpressionMammalianMutationRemoved first ~400 bp from 5' end (N-terminu…PromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HiBiT-CD63
Plasmid#162591PurposeN-terminally HiBiT-tagged human CD63 under CAG promoterDepositorInserthuman CD63 (CD63 Synthetic, Human, human)
UseTagsHiBiTExpressionMammalianMutationPromoterCAG promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry2-mito
Plasmid#164164PurposeExpresses mCherry2-fused with a mitochondria-targeting signal of the ActA gene of Listeria monocytogenes under the control of CAG promoterDepositorInsertmCherry2-mtActA
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Myc-GRM1-IRES-CyRFP1
Plasmid#195355PurposemGluR1-IRES-cyRFP1 under the control of the pCAG promoterDepositorInsertGRM1-IRES-CyRFP1
UseTagsExpressionMammalianMutationPromoterpCAGAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMCS-rybozyme-IRES-CAS9
Plasmid#64668PurposePlasmid encoding multiple cloning site, rybozyme and IRES-CAS9.DepositorInsertsCAS9
HDV ribozyme
UseCRISPRTagsFlag, Internal ribosome entry site, and guide RNA…ExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCBI-CRY2PHR-VPR
Plasmid#192606PurposePlasmid for constitutive expression of CRY2PHR-VPR.DepositorInsertCRY2PHR-VPR
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-CAHS3-mEGFP
Plasmid#205006PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the RvCAHS3-mEGFP gene positioned in between.DepositorInsertRvCAHS3-mEGFP with 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-SAHS1-mEGFP
Plasmid#205007PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.DepositorInsertRvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
VGluT1-SNAPtag
Plasmid#107371PurposeSynaptic vesicle protein VGluT1 fused to the SNAPtag domainDepositorInsertVesicular Glutamate transporter 1 (Slc17a7 Rat)
UseTagsSNAPtag, 104 aa before C termExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-rat Nrxn3beta AS4(-)
Plasmid#58269PurposeExpression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)DepositorInsertNrxn3beta AS4(-) (Nrxn3 Rat)
UseTagsHAExpressionMammalianMutationPromoterCAGAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-VARNAM
Plasmid#115552PurposeRed fluorescent voltage indicator for mammalian expressionDepositorInsertVARNAM
UseTagsGolgi and ER export signalExpressionMammalianMutationPromoterCMV IE94Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-intron
Plasmid#177804PurposeAn intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.DepositorInsertintron
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP
Plasmid#32476PurposeExpresses PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP markerDepositorInsertPSAM Y115F,L141F-5HT3 HC
UseTagsIRES-GFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-eSpCAS9-NG-pA
Plasmid#131466PurposeExpresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized eSpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-rat Nrxn1beta AS4(-)
Plasmid#58267PurposeExpression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)DepositorInsertNrxn1beta AS4(-) (Nrxn1 Rat)
UseTagsHAExpressionMammalianMutationsee commentsPromoterCAGAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-SpCAS9-NG-pA
Plasmid#131465PurposeExpresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized SpCas9-NG
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-miR9/9*
Plasmid#177805PurposeThe pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.DepositorInsertmiR-9/9*
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChETA-mRuby2-ST
Plasmid#109126PurposeCation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChETA-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-rat Nrxn2beta AS4(-)
Plasmid#58268PurposeExpression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)DepositorInsertNrxn2beta AS4(-) (Nrxn2 Rat)
UseTagsHAExpressionMammalianMutationPromoterCAGAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eGtACR1-mRuby2-ST
Plasmid#109136PurposeAnion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendritesDepositorInserteGtACR1-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only