We narrowed to 8,370 results for: construct

1. (829) SB Tet-ON dCasphi-[myc-ZIM3-HA] U6-gStop

   Plasmid

   #195622

   Purpose

   Sleeping Beauty Tet-ON plasmid for induced expression of dCasphi-ZIM3 CRISPRi construct

   Depositor

   Tonia Rex

   Insert

   dCasphi
   

   Use

   Tags

   C-Myc (NLS), HA tag, and ZIM3

   Expression

   Mammalian

   Mutation

   P749A and P753A

   Promoter

   Tight TRE promoter; Tet-ON (doxycycline)

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. (942) pAAV EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

   Plasmid

   #195624

   Purpose

   pAAV EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct

   Depositor

   Tonia Rex

   Insert

   dCasphi
   

   Use

   AAV

   Tags

   C-Myc (NLS), HA tag, and ZIM3

   Expression

   Mammalian

   Mutation

   P749A and P753A

   Promoter

   EF1a (Mlu1/EcoR1)

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Human Coxsackievirus A16 strain G10 2A protease

   Plasmid

   #228632

   Purpose

   Bacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA)

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Enterovirus Coxsackievirus A16 2A protease (HEVAgp1 Coxsackievirus 16)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSLIK DNM1L-V5 neo

   Plasmid

   #220363

   Purpose

   Lentiviral expression vector for an inducible V5-tagged DNM1L construct

   Depositor

   Kevin Janes

   Insert

   DNM1L (DNM1L Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6 - iFAST

   Plasmid

   #209414

   Purpose

   iFAST containing plasmid for endogenous fusion constructs

   Depositor

   Patrick Van Dijck

   Article

   Devos et al Microbiology (Reading). 2024 Mar;170(3).

   Insert

   iFAST
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-MBP-CreM_E352A

   Plasmid

   #112239

   Purpose

   pET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM with E352A mutation

   Depositor

   Emily Balskus

   Article

   Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

   Insert

   CreM_E352A
   

   Use

   Tags

   MBP

   Expression

   Mutation

   Glutamate 352 (E352) mutated to an alanine.

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pexK4_cross

   Plasmid

   #89677

   Purpose

   Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the cross shown in figure 3G in the associated article.

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

   Insert

   cross
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pexK4_drigalski

   Plasmid

   #89676

   Purpose

   Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the Drigalski spatula shown in figure 3E of associated article.

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

   Insert

   drigalski
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pexK4_square

   Plasmid

   #89674

   Purpose

   Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the square shown in figure 3F of associated article.

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

   Insert

   square
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pexK4_wm_ruleB

    Plasmid

    #89675

    Purpose

    Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the three-armed star shown in figure 3D of associated article.

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

    Insert

    wm_ruleB
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTALEN-STAR-T

    Plasmid

    #87522

    Purpose

    Destination vector for TALEN construction

    Depositor

    Steve Pollard

    Article

    Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology and TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTALEN-STAR-A

    Plasmid

    #87523

    Purpose

    Destination vector for TALEN construction

    Depositor

    Steve Pollard

    Article

    Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology and TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTALEN-STAR-C

    Plasmid

    #87524

    Purpose

    Destination vector for TALEN construction

    Depositor

    Steve Pollard

    Article

    Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology and TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTALEN-STAR-G

    Plasmid

    #87525

    Purpose

    Destination vector for TALEN construction

    Depositor

    Steve Pollard

    Article

    Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology and TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTALE-STARVP64-A

    Plasmid

    #87531

    Purpose

    Destination vector for TALE-VP64 construction

    Depositor

    Steve Pollard

    Article

    Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology and TALEN; Tale-tf (activator)

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCASCADE-fabI-gltA1-gltA2-udhA-zwf

    Plasmid

    #87148

    Purpose

    fabI-gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    fabI-gltA1-gltA2-udhA-zwf guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCASCADE-fabI-gltA1-gltA2-udhA-gapAP1

    Plasmid

    #87154

    Purpose

    fabI-gltA1-gltA2-udhA-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    fabI-gltA1-gltA2-udhA-GapAP1 guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. ReaLiSM A1-43-GPIba+2cys

    Plasmid

    #59577

    Purpose

    express VWF A1-GPIba ReaLiSM construct for single molecule study

    Depositor

    Timothy Springer

    Insert

    VWF A1-linker-GPIba (VWF Human)
    

    Use

    Tags

    6His

    Expression

    Mammalian

    Mutation

    Promoter

    chick beta-actin

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

19. AAVS1-DRGFP

    Plasmid

    #113193

    Purpose

    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    DR-GFP
    

    Use

    Donor construct with t2a-hygromycin for gene trap…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET29a-human histone 4-in-One

    Plasmid

    #223237

    Purpose

    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

    Depositor

    Weixing Zhao

    Inserts

    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    

    Use

    Tags

    Dual StrepII, His6, TEV protease site, and VSVG

    Expression

    Bacterial

    Mutation

    A103G

    Promoter

    T7

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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