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pCMV HA hRB delta CDK + T252

Plasmid

#58910

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T252 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAM29 (wt)

Plasmid

#31143

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM29 (ADAM29 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

WT

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-N23PP/G51PEKN

Plasmid

#118583

Purpose

Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

N23 mutated to P23, G51 mutated to P51, a Pro res…

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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ADAP1

Plasmid

#163905

Purpose

Full length mouse ADAP1 for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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ECFP-Bcr NE/AA

Plasmid

#36418

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

breakpoint cluster region (BCR Human)

Tags

ECFP

Expression

Mammalian

Mutation

N689A/ E690A

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
RIPK3 gRNA (BRDN0001147505)

Plasmid

#76996

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK3 (RIPK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RIPK3 gRNA (BRDN0001145972)

Plasmid

#76997

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK3 (RIPK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RIPK3 gRNA (BRDN0001145339)

Plasmid

#76998

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK3 (RIPK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pKD251

Plasmid

#124714

Purpose

Expression of torS under Ptac and torT under PD59_luxR

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

torS (torS )
torT (torS )
lacI (lacI )
luxR

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST40-ARL8B-V5-His6

Plasmid

#67446

Purpose

Mammalian expression of hArl8b with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
P63-sp64 Poly A

Plasmid

#59435

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 63

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
WT MetNI in pBAD

Plasmid

#118254

Purpose

Wild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity Assay

Depositor

Douglas Rees

Article

Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

Inserts

MetN (metN Escherichia coli)
MetI (metI Escherichia coli)

Tags

10x His Tag and Enterokinase Cleavage Site

Expression

Bacterial

Promoter

Arabinose Promoter and None

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Bcr DH domain NE/AA pGEX

Plasmid

#38180

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR DH domain N689A/E690A (BCR Human)

Tags

GST

Expression

Bacterial

Mutation

BCR DH domain N689A/E690A

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
mTFP1-Gamma-Tubulin-17

Plasmid

#55485

Purpose

Localization: Centrosomes, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pSP64-hERG1a F656A

Plasmid

#53054

Purpose

Expresses alpha subunit of F656A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Phenylalanine 656 to Alanine

Promoter

SP6

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a Y652A

Plasmid

#53053

Purpose

Expresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Tyrosine 652 to Alanine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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gh13

Plasmid

#106694

Purpose

expression of gRNA targeting GALNT13

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT13 (GALNT13 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMALp2x-SCO5461(E164D)(43-204)

Plasmid

#42508

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Deleted amino acids 1-42 (nucleotide 1-126); chan…

Promoter

tac

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
P29-sp64 Poly A

Plasmid

#59434

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 29

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pRR-S1

Plasmid

#69998

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

G329T (G110V)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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