We narrowed to 19,001 results for: Tec

1. pCellFree_G03 WAVE 2

   Plasmid

   #67098

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   WAVE 2 (WASF2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-dCas9-PBE

   Plasmid

   #98159

   Purpose

   Expresses CRISPR base editor in maize

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-dCas9-UGI-NLS
   

   Use

   Maize

   Expression

   Plant

   Promoter

   Ubi

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKB083

   Plasmid

   #181952

   Purpose

   IPTG-inducible expression of PhoQ

   Depositor

   Jeffrey Tabor

   Article

   Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

   Insert

   PhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
   

   Expression

   Bacterial

   Promoter

   PhoQ-Ptac; lacI-PlacIq

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Fragment 1 Null

   Plasmid

   #198729

   Purpose

   Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   gRNA expression cassette in pENTR4
   

   Use

   Gateway-compatible entry vector

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmKalama1-Nuc

   Plasmid

   #14894

   Purpose

   Mammalian expression of mKalama1 blue fluorescent protein with NLS

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Insert

   pmKalama1-Nuc
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   I178T mismatch, replace EYFP of the vector pEYFP-…

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

6. p35S-A3A-PBE

   Plasmid

   #119773

   Purpose

   Conversion of C to T in potato

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS-UGI-NLS
   

   Tags

   NLS (flanking UGI)

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   CaMv 2x35S

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL2620 (pTniQ_Vch)

   Plasmid

   #200892

   Purpose

   Expresses human codon-optimized V. cholerae TniQ from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchTniQ
   

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAP003l2

   Plasmid

   #209748

   Purpose

   RepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL bla

   Depositor

   Ahmed Badran

   Article

   Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

   Insert

   NpuC-C[AGGA]LFVY-NpuN-SsrA(rc)
   

   Expression

   Bacterial

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABE8r-R153del

   Plasmid

   #208296

   Purpose

   Expresses ABE8r in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA8r-R153del-SpCas9 D10A
   

   Expression

   Mammalian

   Mutation

   W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. VRER-ABEmax

    Plasmid

    #119812

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (VRER)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSH1/M-FRBl2-E

    Plasmid

    #15289

    Depositor

    David Spencer

    Article

    Li et al Gene Ther. 2002 Feb . 9(4):233-44.

    Insert

    FRAP-rapamycin binding domain (MTOR Human)
    

    Tags

    HA epitope and Myristoylation-targeting domain c-…

    Expression

    Mammalian

    Mutation

    res. 2025-2113, T2098L

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG1280 NLS-mCherry-FIREtag-NES

    Plasmid

    #205172

    Purpose

    expresses NLS-mCherry-FIREtag-NES in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    NLS-mCherry-FIREtag-NES
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB6630

    Plasmid

    #106160

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6371 into Yarrowia lipolytica chromosomal location IntC_3, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. EK0303 SFFV-mCherry-SG(Bdnf.s3)SG-EGFP (FLP-IN)

    Plasmid

    #191180

    Purpose

    Negative control sensor in human cells.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s3:EGFP (negative control)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSplit-CO-hDib1

    Plasmid

    #58788

    Purpose

    Express LucC-hDib1 fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. CP1041-CBEmax

    Plasmid

    #119803

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1041) -UGI-UGI
    

    Expression

    Mammalian

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. UBQ10:sXVE:(MCS)-S11

    Plasmid

    #108179

    Purpose

    Binary vectors used for the inducible expression of tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    UBQ10:sXVE:

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b LFn-Fla

    Plasmid

    #84871

    Purpose

    Bacterial expression of LFn-Fla fusion

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    Fla
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCfB6371

    Plasmid

    #106153

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6630, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CP1249-ABEmax

    Plasmid

    #119809

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (CP1249)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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