We narrowed to 32,724 results for: eng

1. pILGFP4K

   Plasmid

   #104506

   Purpose

   Used to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter gene

   Depositor

   Claudia Vickers

   Article

   Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

   Insert

   SpGAL1 promoter-yEGFP
   

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILGFP4O

   Plasmid

   #104510

   Purpose

   Used to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter gene

   Depositor

   Claudia Vickers

   Article

   Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

   Insert

   SpdGAL2 promoter-yEGFP
   

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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3. pILGFP4P

   Plasmid

   #104511

   Purpose

   Used to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter gene

   Depositor

   Claudia Vickers

   Article

   Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

   Insert

   SptGAL2 promoter-yEGFP
   

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTK_BsmBI_LacZ_Cerulean_Nanotag_60

   Plasmid

   #130528

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   LacZ
   

   Use

   Enhancer cloning nanotag reporter vector

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTK_BsmBI_LacZ_Citrine_Nanotag_45

   Plasmid

   #130525

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   LacZ
   

   Use

   Enhancer cloning nanotag reporter vector

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTK_BsmBI_LacZ_Cherry_Nanotag_106

   Plasmid

   #130547

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   LacZ
   

   Use

   Enhancer cloning nanotag reporter vector

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRH-1328 LV EF1a-CD20 IRES-EGFP

   Plasmid

   #201918

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD20
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI

   Plasmid

   #246754

   Purpose

   RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.

   Depositor

   Wensheng Wei

   Article

   Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

   Insert

   CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI
   

   Use

   Synthetic Biology

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. double cut NH-donor

   Plasmid

   #83576

   Purpose

   eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   eGFP
   

   Use

   Nhej donor

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sg-A

    Plasmid

    #83807

    Purpose

    Express sgRNA targeting SA site

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI

    Plasmid

    #246755

    Purpose

    RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BsaI.

    Depositor

    Wensheng Wei

    Article

    Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

    Insert

    CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI
    

    Use

    Synthetic Biology

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9 (PX165)

    Plasmid

    #48137

    Purpose

    Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3_cAMPFIRE-L

    Plasmid

    #182279

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUltra-U6-gRNAs1-5

    Plasmid

    #178569

    Purpose

    Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

    Depositor

    Christopher Lengner

    Article

    Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

    Insert

    5 gRNA-tRNA array with U6 promoter
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP_mNG2(11)_Clathrin light chain

    Plasmid

    #82608

    Purpose

    Expresses mNG2(11) tagged clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNG2(11)_Clathrin light chain
    

    Expression

    Mammalian

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFW2000

    Plasmid

    #224211

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQAT57

    Plasmid

    #223429

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgRNA 1 GAPDH

    Plasmid

    #83808

    Purpose

    Express Sg-1 sgRNA targeting GAPDH

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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