We narrowed to 19,993 results for: nls
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Plasmid#24367DepositorPublicationInsertQF
ExpressionInsectAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-fDIO-Cre (AAV1)
Viral prep#121675-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T
Plasmid#225062PurposeExpression and Nuclear-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
TagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2
Plasmid#210749PurposeCoding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeatsDepositorPublicationInsertsCjSp D8A Cas9
Bilbo sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD8A, N-terminal Cj Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNeoG2
Plasmid#200222PurposeNeoR nls-mNG marked rapid RMCE integration vectorDepositorTypeEmpty backboneExpressionWormAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCH45 (multiAsCas12a-KRAB lenti)
Plasmid#217330PurposeLentiviral expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPR and LentiviralTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2MutationR1226A/E174R/S542R/K548RPromoterUCOE-SFFVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)
Plasmid#175571PurposeInactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activationDepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE8e-NRCH
Plasmid#165416PurposeExpresses the base editor ABE8e-NRCH in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABE8e-NRCH
TagsBP-NLSExpressionMammalianPromoterCMVAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAGM55261
Plasmid#153210PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DYRK1A/pTRE3G-FL-hXIST
Plasmid#149608PurposeExpress doxycycline-inducible full length of human XIST cDNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertX-inactive specific transcript (XIST Human)
ExpressionMammalianPromoterpTRE3GAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SPACE (pRZ1813)
Plasmid#140242PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSPACE
ExpressionMammalianMutationV82G in TadA*, D10A in Cas9, R187W in pmCDA1PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
LbABE8e
Plasmid#138504PurposeA-to-G base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertecTadA(8e)-dLbCas12a
ExpressionMammalianAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMuLE_ENTR_12GLI-FLuc_R4-R3
Plasmid#113712PurposeSingle pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.DepositorInsert12GLI-FLuc
UseGateway CloningExpressionMammalianAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hRad51(A89E)–Cas9(D10A)
Plasmid#125563PurposeExpresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRad51(A89E)–Cas9(D10A) (RAD51 Human)
UseCRISPRExpressionMammalianAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgRNA
Plasmid#124845PurposeVector for FLP-dependent expression of SaCas9 with gRNADepositorInsertSauCas9 gRNA scaffold
UseAAV, CRISPR, and Mouse TargetingAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
BICstim-Gag-dCAS9-VPR
Plasmid#120922Purposeencodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAG (FMLV)-dCAS9-VPR
TagsnoneExpressionMammalianPromoterhCMVAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLO 62.4
Plasmid#123123PurposeHuman expression vector for deltaproteobacteria CasX and sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCasX protein
CasX sgRNA
UseCRISPRExpressionMammalianAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pV1524
Plasmid#111431PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP
Plasmid#113884PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFPDepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepPromoterpHR-CMV-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFC902
Plasmid#106904PurposeThe vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1DepositorInsertscas9
pyrG
U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by