We narrowed to 25,285 results for: crispr

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sgTP53_3

Plasmid

#78164

Purpose

sgTP53

Depositor

William Hahn

Publication

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pJW1310

Plasmid

#61253

Purpose

Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Publication

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

U6 promoter

Use

CRISPR; Cloning template

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDD317 (Pmyo-2::GFP + SEC)

Plasmid

#73344

Purpose

SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Publication

Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

Insert

Pmyo-2::GFP + SEC

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Promoter

myo-2

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

Plasmid

#92120

Purpose

Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.

Depositor

Ervin Welker

Publication

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MS2-P65-HSF1 (HSF1 Human, Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AICSDP-60: SOX2-mEGFP

Plasmid

#124606

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human SOX2

Depositor

Allen Institute for Cell Science

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SOX2 Homology Arms with linker-mEGFP (SOX2 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available since

May 13, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMK346 (Hygro-P2A-mAID-mCherry)

Plasmid

#121180

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Hygro-P2A-mAID-mCherry

Use

CRISPR

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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hCRISPRi-v2, Stress and Proteostasis (h3), top 5 sgRNAs/gene

Pooled library

#83973

Purpose

Human CRISPRi Pooled Library targeting stress and proteostasis genes

Depositor

Jonathan Weissman

Publication

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

Plasmid

#234826

Purpose

STITCHR R2Tocc editor

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nCas9h840a-xten-R2Tocc

Use

CRISPR

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA Ef1alpha Puro-T2A-GFP

Plasmid

#111596

Purpose

sgRNA Ef1alpha Puro-T2A-GFP

Depositor

Jonathan Weissman

Publication

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pEN243-KH217

Plasmid

#224550

Purpose

Cas9-2A-puro and sgRNA targeting the STOP codon mouse Car2

Depositor

Elphege Nora

Publication

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

Cas9 and sgRNA targeting the STOP codon of mouse CAR2 (Car2 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-mClover3-TDP-43ΔNLS

Plasmid

#214917

Purpose

inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta

Depositor

Ophir Shalem

Publication

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

TDP-43ΔNLS (TARDBP Human)

Use

Lentiviral

Tags

mClover3

Mutation

mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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plRL2

Plasmid

#163631

Purpose

Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

Depositor

Jeremy Rock

Publication

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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px335 Slc1a3 nickase 1

Plasmid

#129410

Purpose

Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

Depositor

Walker Jackson

Publication

Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

Insert

sgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA-BSD

Plasmid

#175583

Purpose

Expresses sgRNA for CasRx in mammalian cell

Depositor

Qi Xie

Publication

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AAV_NLS-SaCas9-NLS-VPR

Plasmid

#68496

Purpose

AAV vector containing SaCas9 fused to VPR

Depositor

George Church

Publication

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

SaCas9-VPR

Use

AAV and CRISPR

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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dCas12Max-ABE

Plasmid

#195335

Purpose

vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Publication

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human codon-optimized dCas12Max-ABE

Expression

Mammalian

Mutation

N243R, E336R, D1049A

Promoter

CBh, CMV, hU6

Available since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMK348 (BSD-P2A-mAID-mClover)

Plasmid

#121182

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BSD-P2A-mAID-mClover

Use

CRISPR

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEJS613_pTetR-P2A-BFPnls/sgTelo

Plasmid

#108649

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Publication

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Telomere-targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hRad51(S208E-A209D)–Cas9(D10A)

Plasmid

#125561

Purpose

Expresses the hRad51(S208E-A209D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Publication

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rad51(S208E A209D)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRDA_078

Plasmid

#158582

Purpose

Lentiviral expression of BE4max and customizable gRNA

Depositor

John Doench, David Root

Publication

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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