We narrowed to 11,195 results for: AGA

1. SpIMPDH

   Plasmid

   #206980

   Purpose

   Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

   Depositor

   Vern Schramm

   Article

   Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

   Insert

   SpIMPDH (guaB Streptococcus pyogenes)
   

   Tags

   His tag (with TEV cleavage site)

   Expression

   Bacterial

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET48 AtGUN1-SMR1

   Plasmid

   #136361

   Purpose

   Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)

   Depositor

   Tatsuru Masuda

   Article

   Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

   Insert

   cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)
   

   Tags

   TRX-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET48 AtGUN1-SMR2

   Plasmid

   #136362

   Purpose

   Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)

   Depositor

   Tatsuru Masuda

   Article

   Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

   Insert

   cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)
   

   Tags

   TRX-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET48 AtGUN1-PPR1

   Plasmid

   #136359

   Purpose

   Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

   Depositor

   Tatsuru Masuda

   Article

   Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

   Insert

   cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)
   

   Tags

   TRX-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET48 AtGUN1-PPR2

   Plasmid

   #136360

   Purpose

   Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

   Depositor

   Tatsuru Masuda

   Article

   Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

   Insert

   cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)
   

   Tags

   TRX-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBW2122_pCAG-PV1-GAI-L1-Bxb1-469C-NLS-BGHpA

   Plasmid

   #108816

   Purpose

   Expresses aa469 to C-terminus of Bxb1 integrase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Bxb1 integrase fragment aa469 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR v2-sgNCKAP1-2

   Plasmid

   #210134

   Purpose

   knock out NCKAP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Nck-associated protein 1 (NCKAP1 Human)
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYL214

   Plasmid

   #171031

   Purpose

   CRISPR plasmids encodes Cas9 and a sgRNA targeting EZH2 exon 2 - intron 2 junction (sequence: GCAGACGAGCTGATGAAGTAA)

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 (EZH2 Human)
   

   Use

   CRISPR

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBW1599_pCAG-PV1-PhiC31-N233-L1-ABI-NLS-BGHpA

   Plasmid

   #108797

   Purpose

   Expresses N-terminus to aa233 of PhiC31 integrase fused to ABI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   PhiC31 integrase fragment N-terminus to aa233
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   ABI,NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBW1604_pCAG-PV1-PYL-L1-PhiC31-234C-BGHpA

    Plasmid

    #108798

    Purpose

    Expresses aa234 to C-terminus of PhiC31 integrase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment aa234 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. CSAC-Crys

    Plasmid

    #164967

    Purpose

    Expresses sgRNA in mammalian cells

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    hU6, hSyn

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBW1349_pCAG-PV1-PhiC31-N571-L1-FRB-NLS-BGHpA

    Plasmid

    #108795

    Purpose

    Expresses N-terminus to aa571 of PhiC31 integrase fused to FRB CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment N-terminus to aa571
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpA

    Plasmid

    #108796

    Purpose

    Expresses aa572 to C-terminus of PhiC31 integrase fused to FKBP CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment aa572 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FKBP and NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW2115_pCAG-PV1-PhiC31-N233-L1-GID1-NLS-BGHpA

    Plasmid

    #108805

    Purpose

    Expresses N-terminus to aa233 of PhiC31 integrase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment N-terminus to aa233
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBW2116_pCAG-PV1-GAI-L1-PhiC31-234C-NLS-BGHpA

    Plasmid

    #108806

    Purpose

    Expresses aa234 to C-terminus of PhiC31 integrase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment aa234 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-SFPQY527A

    Plasmid

    #166961

    Purpose

    Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ-Y527A (SFPQ Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    SFPQ-Y527A

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

    Plasmid

    #86610

    Purpose

    Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid

    #86611

    Purpose

    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEThT-RvCAHS12

    Plasmid

    #192468

    Purpose

    Bacterial expression of RvCAHS12

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS12
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG416GALL-ccdB-6stop

    Plasmid

    #203511

    Purpose

    Gateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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