We narrowed to 19,001 results for: Tec

1. pOCC7

   Plasmid

   #118876

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB

   Plasmid

   #171427

   Purpose

   Expression of HaloTag N58-(GGS)X6-FRB in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-FRB
   

   Expression

   Mammalian

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUPD2_miniDFR (GB2566)

   Plasmid

   #160595

   Purpose

   Minimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR region

   Depositor

   Diego Orzaez

   Article

   Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

   Insert

   miniDFR
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TAL2430

   Plasmid

   #36829

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   HDAC2-TALEN_Left (Hdac2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. Marathon-RT tetramutant for Split-PE (pEH101)

   Plasmid

   #190109

   Purpose

   Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   D14R, D74R, N116K, N197R

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5 HA3-eDHFR-T2A-neo

   Plasmid

   #86396

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A neo
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A neo

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. YSBE 508 : pNEO_GFP

   Plasmid

   #92081

   Purpose

   Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, Neo selection

   Depositor

   Yong-Sun Bahn

   Article

   So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

   Insert

   GFP
   

   Use

   tagging proteins in cryptococcus neoforman

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CP1012-ABEmax

   Plasmid

   #119806

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1012)
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOCC154

   Plasmid

   #118902

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. UBQ10:sXVE:GFP1–9

    Plasmid

    #108187

    Purpose

    Binary vectors used for the inducible expression of tripartite split-sfGFP components (GFP1-9) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Insert

    GFP1-9
    

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    UBQ10:sXVE:

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)

    Plasmid

    #191169

    Purpose

    Expression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:p53(R248Q).s2:EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNW3

    Plasmid

    #53372

    Purpose

    Csy4 and Cas9 D10A nickase expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9n (D10A)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe hygro NFS1

    Plasmid

    #102977

    Purpose

    Expression of NFS1

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    NFS1 (NFS1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Silent mutations to eliminate shNFS1_1 binding si…

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK Lentivirus Single Cassette Destination Vector (pAN2414)

    Plasmid

    #194220

    Purpose

    Backbone for constructing single casette expression plasmids using the Mammalian Tookit

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-TO-Sp dCas9-mVenusN

    Plasmid

    #191396

    Purpose

    SpdCas9 fused to N-terminal part of mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    N-terminal part of split mVenus
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. GWF297

    Plasmid

    #133612

    Purpose

    TET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFP

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    TET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS2386-Tier1-PhCMV-GCaMP6s

    Plasmid

    #169519

    Purpose

    Tier-1 vector encoding PhCMV-driven calcium sensor GCaMP6s (PhCMV-GCaMP6s-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven green fluorescent genetically encoded calcium indicator GCaMP6s
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSMART-GFPuv

    Plasmid

    #65822

    Purpose

    GFPuv Expression, Low Phosphate Dependent E. coli Expression

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    gfpuv
    

    Expression

    Bacterial

    Promoter

    E . coli insulated waaH gene promoter, low phosp…

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOCC153

    Plasmid

    #118901

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    TwinStrep, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

    Plasmid

    #125796

    Purpose

    for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

    Depositor

    P. Chen

    Article

    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

    Insert

    CD40L (lose of function mutant)-mgSrtA
    

    Expression

    Mammalian

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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