167,924 results

1. pcDNA3.1-HaloAKAR2.1-T2A-EGFP

   Plasmid

   #216489

   Purpose

   Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.1
   

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-TORCAR-NLS

   Plasmid

   #64931

   Purpose

   Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR-NLS (EIF4EBP1 Human, Synthetic)
   

   Tags

   Cerulean, Nuclear localization signal (NLS), and …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. ALPaGA

   Plasmid

   #175272

   Purpose

   Lactate biosensor in anaerobic conditions

   Depositor

   Jerome Bonnet

   Article

   Zuniga et al ACS Synth Biol. 2021 Dec 1. doi: 10.1021

   Insert

   LldR_Synthetic_promoter_sfGFP
   

   Expression

   Bacterial

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-AKAR4-Kras

   Plasmid

   #61621

   Purpose

   Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

   Depositor

   Jin Zhang

   Article

   Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

   Insert

   AKAR4-Kras
   

   Tags

   C-terminal targeting sequence from Kras, Cerulean…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. EXT1- D164A-pLJC2-3XFLAG

   Plasmid

   #215828

   Purpose

   Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

   Depositor

   Yoav Shaul

   Article

   Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

   Insert

   Exostosin glycosyltransferase 1 Mutant (EXT1 Human)
   

   Use

   Lentiviral

   Tags

   3X Flag

   Expression

   Mammalian

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6xHis-SNAP-PI(3)P Probe

   Plasmid

   #211508

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   FYVE-Hrs x 2 (PIKFYVE Human)
   

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTcI-Wasabi

   Plasmid

   #86101

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI
   mWasabi
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   pR/pL and pR/pL, LacI

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOlyA(E69A)

   Plasmid

   #132392

   Purpose

   Mutant version of OlyA that senses sphingomyelin without or with cholesterol

   Depositor

   Arun Radhakrishnan

   Article

   Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

   Insert

   Ostreolysin A point mutant (E69A)
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: C62S, E69A, C94S, S151C

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

   Plasmid

   #196678

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   PKIαmut-P2A-mRuby3
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3XFlagNICD2

    Plasmid

    #20184

    Depositor

    Raphael Kopan

    Article

    Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

    Insert

    Notch 2 intracellular domain (Notch2 Mouse)
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Contains murine Notch 2 Intracellular Domain (Met…

    Promoter

    CMV

    Available Since

    Jan. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pet28a_Human_H3.1

    Plasmid

    #42631

    Depositor

    Joe Landry

    Insert

    Human H3.1 (H3C1 Human)
    

    Tags

    Untagged

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAAV-CAG-DIO-mTurquoise2

    Plasmid

    #104059

    Purpose

    AAV expression of Cre-dependent reporter mTurquoise2

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    mTurquoise2
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP_sfCherry2(11)_zyxin

    Plasmid

    #83034

    Purpose

    Expresses sfCherry2(11) tagged zyxin (human) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_zyxin
    

    Expression

    Mammalian

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113341

    Purpose

    Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA

    Plasmid

    #218527

    Purpose

    sgRNA targeting human EIF2AK2/PKR

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK2 (EIF2AK2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-ApoE2

    Plasmid

    #197146

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE2 (APOE Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. E1b-GFP-Tol2

    Plasmid

    #37845

    Depositor

    Nadav Ahituv

    Article

    Birnbaum et al Genome Res. 2012 Jun;22(6):1059-68. Epub

    Type

    Empty backbone

    Use

    Zebrafish enhancer assay vector

    Promoter

    E1b

    Available Since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI-Puro_hCOL4A2-Myc-His-WPRE

    Plasmid

    #198080

    Purpose

    Express hCOL4A2 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A2 (COL4A2 Human)
    

    Tags

    TEV-Myc-His

    Expression

    Mammalian

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKIIa-eArchT3.0-P2A-EGFP-WPRE-hGHpA

    Plasmid

    #51110

    Purpose

    Forebrain principal neuron expression of eArchT3,0 with physically uncoupled EGFP fluorophore

    Depositor

    Jonathan Ting

    Insert

    eArchT3.0
    

    Use

    AAV

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. SnailHA_pcDNA3

    Plasmid

    #31697

    Depositor

    Paul Wade

    Article

    Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66

    Insert

    Snail (SNAI1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. Slug_pGL2

    Plasmid

    #31695

    Depositor

    Paul Wade

    Article

    Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

    Insert

    Slug (SNAI2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAS_4xMma PylT_FLAG-Mma PylRS AF

    Plasmid

    #140023

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y271A, Y349F

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pOpen_taq

    Plasmid

    #153315

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    Drew Endy

    Insert

    Taq polymerase
    

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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24. pET22-HT/plsA

    Plasmid

    #73625

    Purpose

    Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

    Depositor

    James Williamson, T. J. Kappock

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. mEos3.2-C1

    Plasmid

    #54550

    Purpose

    Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson, Tao Xu

    Article

    Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

    Type

    Empty backbone

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. pCEP4-hERG1a

    Plasmid

    #53052

    Purpose

    Expresses alpha subunit of hERG1a K channel in mammalian cells

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. MSCV Luciferase PGK-hygro

    Plasmid

    #18782

    Depositor

    Scott Lowe

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Luciferase and Retroviral

    Expression

    Mammalian

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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28. pP2-P1(S')

    Plasmid

    #196334

    Purpose

    Plasmid expressing chimeric P2-P1(S') tail fiber

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Insert

    chimeric tail fiber P2-P1(S') and chaperone protein U'
    

    Expression

    Bacterial

    Promoter

    P2 late promoter

    Available Since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. v1em-Cterm-PE2max-U6-pegRNA

    Plasmid

    #198733

    Purpose

    AAV genome encoding C-terminal PE2max and U6 expression cassette

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    NpuC-CtermPE2max
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. v1em-Nterm-PE2max-U6-sgRNA

    Plasmid

    #198732

    Purpose

    AAV genome encoding N-terminal PE2max and U6 expression cassette

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    Nterm PE2max-NpuN
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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