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Prohibitin-bio-His

Plasmid

#50800

Purpose

Expresses enzymatically monobiotinylated full length Prohibitin

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

Prohibitin

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pSuper-Rem2-2

Plasmid

#51595

Purpose

shRNA 2 against rodent Rem2

Depositor

Suzanne Paradis

Article

Moore et al PLoS One. 2013 Aug 26;8(8):e74751. doi:

Insert

Rem2 (Rem2, Rem2 Rat, Mouse)

Use

RNAi

Promoter

U6

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
EKAR2G_design1_mTFP_227_Venus_173

Plasmid

#39835

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pBS-L1PA4CHmneo

Plasmid

#69607

Purpose

Expresses a neomycin tagged codon optimized L1PA4

Depositor

Astrid Roy-Engel

Article

Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

Insert

L1PA4

Tags

mneo retrotransposition cassette

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos AA

Plasmid

#8967

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362A, S374A

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pCDH_ss-SBP-EGFP

Plasmid

#172358

Purpose

Reporter to monitoring a streptavidin-binding peptide trafficking

Depositor

Guido Kroemer

Article

Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

Insert

ER importing signal sequence

Use

Lentiviral

Tags

SBP-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pVH72-Tier1-PhCMV-SEAP

Plasmid

#169560

Purpose

Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven human secreted placental alkaline phosphatase

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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SERA1-bio-His

Plasmid

#50812

Purpose

Expresses enzymatically monobiotinylated full length SERA1

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA1

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Dicer2

Plasmid

#14777

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (Dicer1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pJM111

Plasmid

#134162

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC 295-C (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUCSh3-mRFP

Plasmid

#170983

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 1-493

Plasmid

#78581

Purpose

Expression flag-USP19 1-493 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-493 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutated 494 residue to stop codon

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pLMB577

Plasmid

#83778

Purpose

Phenylalanine biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

promoter of RL1860

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin W403A

Plasmid

#45974

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin W403A (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pBS cE1 (5' dbx2 mouse cDNA) [TJ#53]

Plasmid

#16288

Depositor

Thomas Jessell

Article

Pierani et al Cell. 1999 Jun 25. 97(7):903-15.

Insert

5' dbx2 cDNA (Dbx2 Mouse)

Expression

Bacterial

Mutation

dbx2 1->415

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

Plasmid

#172224

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B Flag BimEL(TA)

Plasmid

#23093

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TA) (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Changed Threonine 112 to Alanine

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pCAGGS_2pabPAC

Plasmid

#158968

Purpose

2P-activatable bPAC for transient expression

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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UG75-ADH1-CDS1-3HA

Plasmid

#198596

Purpose

CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CDS1 (CDS1 Budding Yeast)

Tags

3HA

Expression

Yeast

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-ADH1-DGK1-3HA

Plasmid

#198595

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Tags

3HA

Expression

Yeast

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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