Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 26,477 results for: vit;

Showing: 9921 - 9960 of 26477 results

  1. HRH3-DuET

    Plasmid
    #213314
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HRH3 (HRH3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213314-plasmid-map-sequence-id-430910

    Enlarge

  3. PDI-QUAD-V5-pcDNA3.1

    Plasmid
    #153550
    Purpose
    PDI-QUAD was generated from PDI-WT pcDNA3.1 (+) using two native active sites (CGHC, CGHC) tagged with V5, and mutated (to SGHS, SGHS) using site directed mutagenesis.
    Depositor
    Julie Atkin
    Article
    Parakh et al iScience. 2020 May 22;23(5):101097. doi:
    Insert
    Protein disulphide isomerase
    Tags
    V5 tag
    Mutation
    C53S, C56S, C397S, C400S
    Promoter
    pCMV
    Available Since
    Aug. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    153550-plasmid-map-sequence-id-308584

    Enlarge

  4. pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

    Plasmid
    #115161
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.
    Inserts
    PTRE - tet activator responsive promoter
    flex sequence
    hM3dq-mCherry (inverted)
    flex sequence
    Use
    AAV
    Tags
    hM3Dq-mCherry
    Expression
    Mammalian
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115161-plasmid-map-sequence-id-220939

    Enlarge

  8. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid
    #190598
    Purpose
    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    mCherry - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190598-plasmid-map-sequence-id-379953

    Enlarge

  11. CCR10-DuET

    Plasmid
    #213197
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR10 (CCR10 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213197-plasmid-map-sequence-id-430761

    Enlarge

  14. pCAT527

    Plasmid
    #180514
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180514-plasmid-map-sequence-id-359727

    Enlarge

  16. pC1-oROS-HT-CaaX

    Plasmid
    #216417
    Purpose
    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.02.06.579232
    Insert
    oROS-HT-CaaX
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216417-plasmid-map-sequence-id-434853

    Enlarge

  18. pAAV-TRE-DIO-Casp3-TEV

    Plasmid
    #183766
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    tetO promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183766-plasmid-map-sequence-id-361683

    Enlarge

  20. CXCR1-DuET

    Plasmid
    #213218
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR1 (CXCR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213218-plasmid-map-sequence-id-430780

    Enlarge

  21. CCR3-DuET

    Plasmid
    #213199
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR3 (CCR3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213199-plasmid-map-sequence-id-430762

    Enlarge

  22. pAR100

    Plasmid
    #180377
    Purpose
    pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).
    Depositor
    Alec Redwood
    Article
    Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:
    Inserts
    NFAT-Luciferase
    hCD8A
    Expression
    Bacterial and Mammalian
    Promoter
    hEF1-HTLV and minimal promoter and an NFAT respon…
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    180377-plasmid-map-sequence-id-370772

    Enlarge

  23. DRD1-DuET

    Plasmid
    #213227
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    DRD1 (DRD1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213227-plasmid-map-sequence-id-431800

    Enlarge

  24. GPR77-DuET

    Plasmid
    #213291
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR77 (C5AR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213291-plasmid-map-sequence-id-430836

    Enlarge

  25. pHRIG-AktDN

    Plasmid
    #53597
    Purpose
    lentiviral expression of dominant negative mouse Akt1 and EGFP
    Depositor
    Heng Zhao
    Article
    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Lentiviral
    Tags
    HA and IRES-EGFP
    Expression
    Mammalian
    Mutation
    Dominant negative (K179M)
    Promoter
    CMV
    Available Since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1

    Plasmid
    #176250
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozym
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR and Synthetic Biology; Expression in micro…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176250-plasmid-map-sequence-id-346579

    Enlarge

  27. pDisplay-oROS-HT

    Plasmid
    #216416
    Purpose
    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.02.06.579232
    Insert
    pDisplay-oROS-HT
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216416-plasmid-map-sequence-id-430079

    Enlarge

  33. GPR123-DuET

    Plasmid
    #213262
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR123 (ADGRA1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213262-plasmid-map-sequence-id-430812

    Enlarge

Showing: 9921 - 9960 of 26477 results