We narrowed to 15,922 results for: nol

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pCellFree_G03 TCF12

Plasmid

#67113

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF12 (TCF12 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Tet-O-FUW-Zfp536

Plasmid

#45845

Depositor

Marius Wernig

Article

Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

Insert

Zfp536 (Zfp536 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet-ON

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pBLO1806_Cas9_2xNLS_human

Plasmid

#74493

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Tags

t2a mCherry

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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apt-CBE-3'end

Plasmid

#219939

Purpose

Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

MCP_32-aa_evo-rA1_2xUGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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apt-ABE8e

Plasmid

#219937

Purpose

Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Com_93-aa_TadA8e

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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IVT-MCP-MMLV-RT

Plasmid

#207457

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-RT

Expression

Bacterial

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pJ211_WT_low

Plasmid

#128848

Purpose

Low copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin B2

Plasmid

#10911

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin B2 (CCNB2 Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pMR-A-6

Plasmid

#138520

Purpose

P3d

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pYL322d1/eGFP-C

Plasmid

#183152

Purpose

Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFW11-ffluc

Plasmid

#128487

Purpose

Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

firefly luciferase gene

Use

Allelic replacement vector

Tags

spy0535 downstream flanking region and spy0535 up…

Promoter

synthetic

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMK408 (mAID-Nluc Hygro)

Plasmid

#121198

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-Nluc Hygro

Use

CRISPR

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-Y186F-T2A-BFP

Plasmid

#194931

Purpose

LV construct encoding Y186F mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB6574

Plasmid

#106132

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTKhc-yibDp-GFP-cp6

Plasmid

#134938

Purpose

Low phosphate inducible circular permutation of GFP

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPcp6

Tags

His tag

Expression

Bacterial

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB4787

Plasmid

#106147

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-I-SceI

Plasmid

#165041

Purpose

Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

I-SceI

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-TKNeo-L2

Plasmid

#141008

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

TKNeo

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D1

Plasmid

#8962

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin D1 (CCND1 Human)

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pMJA180

Plasmid

#128538

Purpose

Expresses E coli Bir A protein

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

E coli BirA

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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