We narrowed to 8,646 results for: AAT

1. pY71-PT7-RiboJ-sfGFP-MGapt

   Plasmid

   #129119

   Purpose

   Expresses a fluorescent reporter for the quantification of cell-free protein expression.

   Depositor

   Matthew Lux

   Article

   Cole et al ACS Synth Biol. 2019 Sep 20;8(9):2080-20

   Insert

   Superfolder GFP with Malachite Green aptamer
   

   Use

   Synthetic Biology

   Tags

   His6 and RiboJ Insulator

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRII-Topo U6-SpAi9L-U6SpDMDL

   Plasmid

   #78608

   Purpose

   Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   U6-SpAi9L-U6SpDMDL
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

3. L4440_BioBrick-hsf-1-sgRNA-B

   Plasmid

   #177787

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hsf-1 (hsf-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

   Plasmid

   #172670

   Purpose

   For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

   Insert

   SDHB pegRNA and SDHB_nick-sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP

   Plasmid

   #172668

   Purpose

   For expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

   Insert

   ALDOB pegRNA and ALDOB_nick-sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

   Plasmid

   #172669

   Purpose

   For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

   Insert

   RNF2 pegRNA and RNF2_nick-sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUDP010

   Plasmid

   #101166

   Purpose

   E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   HH-gRNA-HDV targetting SeILV6 in S. pastorianus
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3Luc-5XNF-kappaB

   Plasmid

   #185695

   Purpose

   Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

   Depositor

   Esther López-Bayghen

   Article

   Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

   Insert

   NF-kappaB (NFKB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   NF-kappaB binding site

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLCHKO_HPRT1_exon2_1_Lb

   Plasmid

   #155051

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of HPRT1 exon 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIRES2-EGFP-p53 WT

    Plasmid

    #49242

    Purpose

    expresses human wt p53 and EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ7854 pHR (hU6-crGLY-EFS-PuroR-WPRE)

    Plasmid

    #214884

    Purpose

    Lentiviral vector encoding RfxCas13d targeting GLY guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crGLY-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3.1-CIB1-Myc

    Plasmid

    #32505

    Depositor

    Nora Heisterkamp, John Groffen

    Article

    Haataja et al J Biol Chem. 2002 Mar 8;277(10):8321-8.

    Insert

    CIB1 (CIB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1(-) rat C/EBP alpha

    Plasmid

    #12550

    Depositor

    Peter Johnson

    Article

    Miller et al J Biol Chem. 2003 Apr 25;278(17):15178-8

    Insert

    CCAAT/Enhancer-binding Protein alpha (Cebpa Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA 3.1(-) mouse C/EBP gamma

    Plasmid

    #12560

    Depositor

    Peter Johnson

    Article

    Parkin et al J Biol Chem. 2002 Jun 28;277(26):23563-7

    Insert

    CCAAT enhancer binding protein gamma (Cebpg Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

15. OR6K6_Deletion_gRNA3

    Plasmid

    #195197

    Purpose

    dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    OR6K6 dual gRNA (OR6K6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. U6-sgRNA-hcr5

    Plasmid

    #193661

    Purpose

    Expression of tandem pre-sgRNA array hcr5 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-DNMT3B-sgRNA-KLF4-sgRNA-TET1-sgRNA-PRR5L-sgRNA-CFTR-sgRNA-tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLCHKO_HPRT1_exon2_1_As

    Plasmid

    #155055

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PAK7 gRNA (BRDN0001149524)

    Plasmid

    #75715

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PAK7

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PAK7 (PAK5 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Circular 200,100 with interspersed loops (FANCC)

    Plasmid

    #180192

    Purpose

    AAV vector carrying a guide RNA targeting the human FANCC mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUDP012

    Plasmid

    #101167

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge