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p207-Switch-ON (FRT)

Plasmid

#217886

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-UMI

Plasmid

#222694

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

PGK without BsmBI cutsite

Promoter

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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p206-Switch-ON

Plasmid

#217885

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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p213-SWITCH-OFF

Plasmid

#217888

Purpose

Retroviral Switch-OFF vector for sgRNA expression; U6-BbsIx2-SWITCH-OFF-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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p205-5'-Switch-ON

Plasmid

#217884

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p204-U6-Switch-ON

Plasmid

#217883

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-TripleColour-StAR

Plasmid

#222695

Purpose

Competition assay

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP, miRFP703, dTomato

Expression

Mammalian

Mutation

PGK, TagBFP, dTomato without BsmBI cutsite

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRetro-UMI

Plasmid

#222686

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

Type

Empty backbone

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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HB101 endA::frt

Bacterial Strain

#45473

Depositor

Michele Swanson

Article

Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

Bacterial Resistance

Streptomycin

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
MM39

Bacterial Strain

#42894

Depositor

Bryan Berger

Article

Su et al J Biol Chem. 2012 Jul 22.

Bacterial Resistance

Streptomycin

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
EcNR2 Strain

Bacterial Strain

#26931

Depositor

George Church

Article

Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

Bacterial Resistance

Chloramphenicol and ampicillin

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
HB101 endA::frt lambda pir

Bacterial Strain

#45472

Depositor

Michele Swanson

Article

Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

Bacterial Resistance

Streptomycin

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
CLM24 ∆lpxM

Bacterial Strain

#132780

Purpose

CLM24 strain with lpxM deletion

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Bacterial Resistance

None

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only
EcNR1 Strain

Bacterial Strain

#26930

Depositor

George Church

Article

Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

Bacterial Resistance

Ampicillin

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
B-95.ΔAΔfabR

Bacterial Strain

#197934

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only
B-95.ΔA

Bacterial Strain

#197933

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only
BL21ΔCF

Bacterial Strain

#102265

Purpose

ompC, ompF double knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

None

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

Bacterial Strain

#99247

Purpose

LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

Depositor

Jeff Hasty

Article

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Bacterial Resistance

Tetracycline

Available since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only
BL21ΔA

Bacterial Strain

#102256

Purpose

ompA deletion strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

None

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
BL21ΔAC

Bacterial Strain

#102261

Purpose

ompA, ompC double knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

None

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only