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eIF3b sgRNA-1 PX459 plasmid

Plasmid

#192249

Purpose

expressing sgRNA-1 targeting eIF3b locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3b locus closed to stop coden (EIF3B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 C-V5-tag ORF7a

Plasmid

#179971

Purpose

Mammalian expression vector for SARS-CoV-1 ORF7a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 ORF7a (ORF7a Synthetic)

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP7 C-V5-tag

Plasmid

#179963

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp7, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 Nsp7 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCPF101-pfQC-cyclase dead

Plasmid

#184878

Purpose

expresses soluble plasmodium falciparum QC protein with a histag, cyclase dead mutant

Depositor

Ferenc Scheeren

Article

Kolli et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

pfQC-cyclase dead

Tags

His tag

Expression

Bacterial

Mutation

F77A & Q79A

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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SOD1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176964

Purpose

Mammalian expression vector encoding SOD1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 ORF7a C-V5-tag

Plasmid

#179989

Purpose

Mammalian expression vector for RaTG13 ORF7a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 ORF7a

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 NSP16 C-V5-tag

Plasmid

#179985

Purpose

Mammalian expression vector for RaTG13 Nsp16, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 Nsp16

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 ORF6 C-V5-tag

Plasmid

#179970

Purpose

Mammalian expression vector for SARS-CoV-1 ORF6, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 ORF6 (ORF6 Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 E C-V5-tag

Plasmid

#179966

Purpose

Mammalian expression vector for SARS-CoV-1 E, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 E (E Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP16 C-V5-tag

Plasmid

#179965

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp16, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 Nsp16 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pOmnibac_ZZ_linker_K490_GFP_SNAP

Plasmid

#159721

Purpose

Expresses kinesin-1 (amino acids 1-490) in Sf9 cells with a C-terminal GFP and SNAP tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

KIF5B (only amino acids 1-490), K490 (KIF5B Human)

Tags

GFP and SNAP tags and ZZ affinity tag

Expression

Insect

Mutation

Only amino acids (1-490)

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pOmnibac_ZZ_linker_ybbR_MAP7_N

Plasmid

#159728

Purpose

Expresses MAP7 (amino acids 1-316) in Sf9 cells with a ZZ affinity tag and a ybbR tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

MAP7 (only amino acids 1-316)(includes MTBD and P123 domains) (MAP7 Human)

Tags

ZZ affinity tag and ybbR tag

Expression

Insect

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC35B1_STOP

Plasmid

#161265

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35B1 (SLC35B1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2plus-TDP-43

Plasmid

#162608

Purpose

Allows for transcription of TDP-43 mRNA for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV ShadowG-AURKA K162M-mTurq2

Plasmid

#157769

Purpose

Expression of AuroraA kinase-dead biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and shadowG

Expression

Mammalian

Mutation

AURKA K162M is a kinase-dead version of AURKA

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV ShadowY-AURKA K162M-mTurq2

Plasmid

#157771

Purpose

Expression of AuroraA kinase-dead biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and shadowY

Expression

Mammalian

Mutation

AURKA K162M is a kinase-dead version of AURKA

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgMARK3

Plasmid

#138706

Purpose

Expresses a human MARK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK3 human (MARK3 Human)

Use

Lentiviral

Promoter

hU6

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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RCH1:HDT1,HDT2-RNAi

Plasmid

#108446

Purpose

Arabidopsis transformation, RNAi vector targeting HDT1/HDT2 with RCH1 promoter

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Inserts

HDT1 (HDA3 Mustard Weed)
HDT2 (HD2B Mustard Weed)

Use

RNAi

Expression

Plant

Promoter

RCH1

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3-CARD14-L156P

Plasmid

#69918

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

L156P

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

Plasmid

#125712

Purpose

Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

eOPN3

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

short version of αCamKinase II promoter (0.4kb)

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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