We narrowed to 166,096 results for: addgene

1. AAV-CAG-flex-GFP-4x6T

   Plasmid

   #196418

   Purpose

   Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   GFP
   

   Use

   AAV and Cre/Lox; Astrocyte-selective

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-loxp-DsRed-loxp-eGFP

   Plasmid

   #141148

   Purpose

   lentiviral expression vector to generate a hypoxia fate-mapping system

   Depositor

   Daniele Gilkes

   Article

   Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

   Insert

   loxp-DsRed-loxp-eGFP
   

   Use

   Lentiviral

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPRia-v2

   Plasmid

   #84832

   Purpose

   CRISPRi/a V2 library parental plasmid

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Inserts

   sgRNA
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-cGAS-HA

   Plasmid

   #130910

   Purpose

   Expresses human cGAS-HA; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   cGAS
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p300 KAT LIC 1M

   Plasmid

   #233587

   Purpose

   Bacterial expression of p300 KAT enzyme

   Depositor

   Scott Showalter

   Article

   Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

   Insert

   p300 KAT (EP300 Human)
   

   Tags

   6xHis MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas9 sgRNA vector

   Plasmid

   #68463

   Purpose

   Cas9 sgRNA vector for cloning

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEM047 [T7-assisted CROPseq sgRNA expression vector]

   Plasmid

   #224899

   Purpose

   Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

   Depositor

   Thomas Norman

   Article

   Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi

   Inserts

   eGFP
   LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 shPIK3CD.v1 puro

   Plasmid

   #58706

   Purpose

   Lentiviral shRNA vector for knockdown of human PIK3CD

   Depositor

   Kevin Janes

   Article

   Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

   Insert

   shPIK3CD (PIK3CD Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pU6-mir451 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164792

   Purpose

   Express miR-451 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   microRNA 451a (MIR451A Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSP1830

    Plasmid

    #70708

    Purpose

    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Insert

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    CAG

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFYF1548 EMX1

    Plasmid

    #47508

    Purpose

    human gRNA expression vector targeting EMX1

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EMX1 (EMX1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. MAC_C_PTH2

    Plasmid

    #111674

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    PTH2 (PTRH2 Human)
    

    Expression

    Mammalian

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4xHRE-MinTK-CRE-ODD

    Plasmid

    #141147

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    4xHRE-MinTK-CRE-ODD
    

    Use

    Lentiviral

    Mutation

    contains an altered Cre gene modified by the addi…

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. BPK3079

    Plasmid

    #78741

    Purpose

    Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    AsCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. murine TCR OTI-2A.pMIG II

    Plasmid

    #52111

    Purpose

    retroviral expression of both TCRalpha and TCRbeta using a single vector

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Inserts

    T cell receptor alpha chain (Tcra Mouse)
    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Retroviral

    Tags

    T2A (from Thosea asigna virus)

    Expression

    Mammalian

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. GOLPH3 shRNA

    Plasmid

    #163860

    Purpose

    shRNA targeting the coding sequences of GOLPH3

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    golgi phosphoprotein 3 (GOLPH3 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TAL3216

    Plasmid

    #42679

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-atg12-left (atg12 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pFGC5941-PacI-ath-STTM832-5p

    Plasmid

    #84163

    Purpose

    target miRNA832-5p for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM832-5p
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFGC5941-PacI-ath-STTM832-3p

    Plasmid

    #84162

    Purpose

    target miRNA832-3p for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM832-3p
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. p65-mNeonGreen

    Plasmid

    #127172

    Purpose

    Fluorescently tagged p65 reporter

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Insert

    GFP-RelA (RELA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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