We narrowed to 13,374 results for: otos

1. CALID

   Plasmid

   #169708

   Purpose

   Cre-activated light-inducible Dre recombinase

   Depositor

   Dali Li

   Article

   Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

   Insert

   a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1_puro_CAG_HexaPro

   Plasmid

   #164077

   Purpose

   Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus

   Depositor

   Yannick Doyon

   Article

   Rivest et al protocols.io 2021

   Insert

   SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   CRISPR

   Tags

   2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

   Expression

   Mammalian

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   CAG

   Available since

   Jan. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. mCerulean3-Caveolin-C-10

   Plasmid

   #55404

   Purpose

   Localization: Membrane, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   Caveolin (CAV1 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pJH4169

   Plasmid

   #200108

   Purpose

   Prig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of snb GFP

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   snb-1 (snb-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Prig-3

   Available since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pTS116 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199369

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-SUMOstar-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

   Plasmid

   #139485

   Purpose

   PCR template for Halo-Tag gene knock-in donor vector

   Depositor

   Kazuhiro Aoki

   Article

   Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

   Insert

   HaloTag-loxP-P2A-dTKneo-pA-loxP
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST_DSUP_RAMVA (pBS0353)

   Plasmid

   #185189

   Purpose

   For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DSUP_RAMVA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pvha6-AgDD

   Plasmid

   #80626

   Purpose

   create protein aggregates in C. elegans

   Depositor

   Thomas Wandless

   Article

   Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

   Insert

   NES-L106P-sfGFP (FKBP1A Human)
   

   Use

   Tags

   MLALKLAGLDI

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNAIIIB cdc42 QL

   Plasmid

   #61636

   Purpose

   mammalian expression of cdc42 constitutively active mutant

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   Cdc42 (CDC42 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q61L, constitutively active

   Promoter

   CMV

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

10. p3E-V5-mScarlet-I_SV40pA (JDW 968)

    Plasmid

    #224529

    Purpose

    A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mScarlet-I
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-mApg5

    Plasmid

    #22958

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

    Insert

    autophagy related genes 5 (Atg5 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

    Plasmid

    #113890

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid

    #128404

    Purpose

    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Exotoxin A (toxA P. aeruginosa)
    

    Use

    Tags

    6xHis tag and DsbA signal sequence for periplasmi…

    Expression

    Bacterial

    Mutation

    242DNNNS246 and 384DQNRT388 engineered glycosylat…

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti2-EF1a-ChR2-EYFP-WRPE

    Plasmid

    #125255

    Purpose

    3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Roger D. Kamm

    Article

    Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

    Insert

    hChR2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSCV-human CSFI-R-L301S-IRES-GFP

    Plasmid

    #133744

    Purpose

    retroviral expression of CSF1-R mutant

    Depositor

    Martine Roussel

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    CSF1-R (CSF1R Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L301S

    Promoter

    MSCV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-DIO-LckAPEX-P2A-EGFP

    Plasmid

    #182826

    Purpose

    Cre-dependent expression of membrane localized APEX

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    LckAPEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUB-nHyPer2 (NLS:HyPer2:NLS)

    Plasmid

    #84730

    Purpose

    Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    HyPer2 targeted to nucleus
    

    Use

    Tags

    NLS (nuclear localisation signal and NLS (nuclear…

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas4_1_2_VA

    Plasmid

    #186438

    Purpose

    Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

    Plasmid

    #113889

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

    Plasmid

    #199355

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA-SUMOstar

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge