We narrowed to 7,746 results for: pac

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pRSFDuet-1_mNeongreen-Erythropoietin

Plasmid

#182422

Purpose

Bacterial expression of mNeongreen-Erythropoietin fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-eythropoietin (EPO Human)

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-C7

Plasmid

#194591

Purpose

Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

C7 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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ABE8.13-m

Plasmid

#136296

Purpose

expresses ABE8.13-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.13-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

Plasmid

#117005

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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ESR1-luciferase

Plasmid

#113351

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-IpgD

Plasmid

#183666

Purpose

N-terminally tagged Shigella flexneri IpgD for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Ldha

Plasmid

#175939

Purpose

knockout mouse Ldha

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Ldha gRNA (Ldha Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b

Plasmid

#182276

Purpose

Expresses recombinant BrCas12b

Depositor

Piyush Jain

Article

Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis, T7, Thrombin site

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE7.8

Plasmid

#102917

Purpose

expresses ABE7.8 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE7.8

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-DIO-TRPV1-P2A-mCherry

Plasmid

#200831

Purpose

In the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherry

Depositor

Hong Chen

Article

Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

Insert

TRPV1-P2A-mCherry (Trpv1 Rat)

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-StefinA

Plasmid

#182419

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-stefin (CSTA Human)

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-INPP4B-CAAX

Plasmid

#116857

Purpose

plasma membrane-targetted PI(3,4)P2 4-phosphatase

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

INPP4B (INPP4B Human)

Tags

HRAS(172-189) and TagBFP2

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

Plasmid

#232244

Purpose

Mammalian expression of N581K RLIM-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

N581K RLIM-GFP-IRES-mCherry (RLIM Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

N581K

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro GFP-REX1[degron]-IRES-mCherry

Plasmid

#232240

Purpose

Mammalian expression of REX1[degron]-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

REX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)

Tags

AcGFP1

Expression

Mammalian

Mutation

REX1 (aa 70-85)

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFIN-RK-GFP-mIRBP156-CHER-WPRE

Plasmid

#44351

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Inserts

RK-GFP
mIRBP156-CHER

Use

Lentiviral

Promoter

human rhodopsin kinase (pos 2554-2850) and mouse …

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pY003 (pFnCpf1_delta Cas)

Plasmid

#69974

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes

Use

CRISPR

Expression

Bacterial

Mutation

Cas genes are deleted from locus

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2A

Plasmid

#202732

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2A (PIP4K2A, FKBP1A Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2B

Plasmid

#202734

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A (3-108):[GGSA]4GG:PIP4K2B (PIP4K2B, FKBP1A Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-L17-FZY2-S100N-T26I&T77I-UGI-Puro

Plasmid

#209645

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

L17-FZY2-S100N-T26I&T77I

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-TRPV1-P2A-DsRed

Plasmid

#200830

Purpose

Transduce all neuron types with AAV to express TRPV1 along with a DsRed fluorescence reporter

Depositor

Hong Chen

Article

Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

Insert

TRPV1-P2A-DsRed (Trpv1 Rat)

Use

AAV

Expression

Mammalian

Promoter

Syn

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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