We narrowed to 13,665 results for: crispr cas9

1. pJAT56

   Plasmid

   #204300

   Purpose

   Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-EGFP
   

   Use

   CRISPR

   Promoter

   3XP3

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ141D

   Plasmid

   #69293

   Purpose

   Gateway entry vector; single gRNA under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDB4283

   Plasmid

   #98702

   Purpose

   A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   gRNA PCR template
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMiniT:SEP-beta2 TKIT donor

   Plasmid

   #169436

   Purpose

   Donor DNA for TKIT knockin of SEP to Gabrb2 in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ148

   Plasmid

   #69300

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 8 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMiniT:SEP-GluA3 TKIT donor

   Plasmid

   #169425

   Purpose

   Donor DNA for TKIT knockin of SEP to GluA3 in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB8792

   Plasmid

   #126910

   Purpose

   Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   ADE2 gRNA
   

   Use

   Synthetic Biology

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ147

   Plasmid

   #69299

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

   Plasmid

   #196710

   Purpose

   Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMLS234

    Plasmid

    #73682

    Purpose

    SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    GFP + Cbr-unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. L1p6_barley_sgRNA_scaffold

    Plasmid

    #231033

    Purpose

    Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 6

    Depositor

    Wilma van Esse

    Article

    Winkelmolen et al bioRxiv 2024.08.22.609225

    Insert

    Scaffold for Cas9 mediated CRISPR
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TaU6

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. L1p5_barley_sgRNA_scaffold

    Plasmid

    #231032

    Purpose

    Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 5

    Depositor

    Wilma van Esse

    Article

    Winkelmolen et al bioRxiv 2024.08.22.609225

    Insert

    Scaffold for Cas9 mediated CRISPR
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TaU6

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. L1p4_barley_sgRNA_scaffold

    Plasmid

    #231031

    Purpose

    Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 4

    Depositor

    Wilma van Esse

    Article

    Winkelmolen et al bioRxiv 2024.08.22.609225

    Insert

    Scaffold for Cas9 mediated CRISPR
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TaU6

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. L1p3_barley_sgRNA_scaffold

    Plasmid

    #231030

    Purpose

    Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 3

    Depositor

    Wilma van Esse

    Article

    Winkelmolen et al bioRxiv 2024.08.22.609225

    Insert

    Scaffold for Cas9 mediated CRISPR
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TaU6

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

    Plasmid

    #99696

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

    Depositor

    George Church

    Insert

    dCas9 and gRNA targeting Afp (Afp Synthetic)
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti

    Plasmid

    #138312

    Purpose

    Lenti expression of AcrIIA14, C-terminus

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA14, C terminus
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRG2

    Plasmid

    #104174

    Purpose

    sgRNA expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. LRG2.1-BFP

    Plasmid

    #120577

    Purpose

    Lentiviral plasmid to co-express a guide RNA and EBFP.

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-Puro-ccdB

    Plasmid

    #82580

    Purpose

    A modified version of plasmid pX330 (#42230): Added are a ccdB stuffer and a Puromycin selection cassette

    Depositor

    Ivo Huijbers

    Article

    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBLO5.01

    Plasmid

    #121418

    Purpose

    pUC19_BsaI-dCas9-BsaI

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    dcas9
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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