We narrowed to 10,370 results for: Pol;

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pLentiCas9-GFP

Plasmid

#78546

Purpose

Fusion protein Cas9+GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

Fusion protein with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-scCAK1

Plasmid

#177014

Purpose

Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)

Tags

Polyhistidine-TEV

Expression

Insect

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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L1-neo-TET

Plasmid

#51284

Purpose

expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

L1RE1 (L1RE1 Human)

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-Rpl22-HA

Plasmid

#111811

Purpose

Express Rpl22HA in astrocytes

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Ribosomal protein L22 (Rpl22 Mouse)

Tags

HA

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1 Raptor GFP

Plasmid

#112744

Purpose

express Raptor-GFP

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Raptor-GFP (RPTOR Human)

Use

Lentiviral

Tags

eGFP

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D rM3D mCherry SV40

Plasmid

#92285

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

rM3D

Use

AAV

Tags

mCherry

Promoter

GfaABC1D

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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LentiV_Cas9_Blast

Plasmid

#125592

Purpose

Lentiviral expression of spCas9 with blasticidin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pFW2500

Plasmid

#224213

Purpose

Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1 and recT

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-GFP

Plasmid

#78548

Purpose

Cas9 protein with GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCARGO-RMCE-Xist

Plasmid

#233268

Purpose

CARGO plasmid containing ~22kb dox-inducible Xist transgene

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Insert

22kb Xist gene, including introns

Expression

Mammalian

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 human cofilin WT

Plasmid

#50859

Purpose

expresses human cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pOPINK-TRABID (NZF1, aa 1-33)

Plasmid

#65437

Purpose

Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

TRABID (ZRANB1 Human)

Tags

His6-GST

Expression

Bacterial

Mutation

only codes for amino acids 1-33 of TRABID, which …

Promoter

T7

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pOPINS-AREL1

Plasmid

#66710

Purpose

Expresses AREL1 HECT domain in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

KIAA0317 (AREL1 )

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pBP-T7_RBS-His6-Thrombin

Plasmid

#72979

Purpose

T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

T7+RBS+His6+thrombin

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
peGFP C3-SPG20

Plasmid

#218578

Purpose

Expresses GFP-tagged human SPG20 in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EGFP-IRES-Neo

Plasmid

#128660

Purpose

mammalian expression of EGFP with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

EGFP

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Kp-1

Plasmid

#163143

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV Puro SCRIB WT

Plasmid

#88886

Purpose

SCRIB WT in MSCV backbone

Depositor

Senthil Muthuswamy

Article

Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

Insert

SCRIB (SCRIB Human)

Use

Retroviral

Tags

N/A

Mutation

N/A

Promoter

MSCV promoter

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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GAPDH-CPG-TV

Plasmid

#70148

Purpose

a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

Depositor

Rudolf Jaenisch

Article

Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

Insert

5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh

Use

Mouse Targeting and Synthetic Biology

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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sAB-K29

Plasmid

#204735

Purpose

synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin

Depositor

Minglei Zhao

Article

Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

Insert

synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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