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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,286 results for: Pol;

Showing: 5081 - 5100 of 9286 results

  1. C-BLa 2.0

    Plasmid
    #92060
    Purpose
    The C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.
    Depositor
    Dieter Langosch
    Article
    Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836
    Insert
    C-BLa 2.0 fusion protein
    Tags
    Flag (DYKDHDG)
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92060-plasmid-map-sequence-id-181586

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  7. pENV8(GAAA)-GFPuv

    Plasmid
    #70128
    Purpose
    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop
    Depositor
    Robert Batey
    Article
    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:
    Insert
    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70128-plasmid-map-sequence-id-130022

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  13. pWL502-crRNA

    Plasmid
    #174383
    Purpose
    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    a crRNA targeting the template strand
    Use
    CRISPR; Archaeal expression
    Expression
    Bacterial
    Promoter
    PhaR promoter
    Available Since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174383-plasmid-map-sequence-id-343840

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  15. pTU2S-b (colE1 origin)

    Plasmid
    #74092
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74092-plasmid-map-sequence-id-141928

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  17. pGBW-m4046940

    Plasmid
    #149261
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149261-plasmid-map-sequence-id-296392

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Showing: 5081 - 5100 of 9286 results