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pRECMYC-Ebu1

Plasmid

#240713

Purpose

pLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al bioRxiv 2025.06.16.660010

Insert

Ebu1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-hIFITM3-Y20A

Plasmid

#58462

Purpose

Expresses human IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (IFITM3 Human)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 to alanine

Promoter

CMV IE

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
Double UP mNeon to mScarlet Rac1-V12

Plasmid

#125136

Purpose

Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Insert

mNeon/mScarletP2A Rac1-V12

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet. 12th AA in Rac1 ch…

Promoter

CAG

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP-VPR

Plasmid

#183936

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP-VPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-PXN

Plasmid

#227315

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of PXN (PXN Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV E2F1 E132

Plasmid

#24224

Purpose

Mammalian expression of human E2F1 (DNA binding mutant) with HA tag

Depositor

Kristian Helin

Article

Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

Insert

E2F1 E132 (E2F1 Human)

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pLV-CMV-tagRFP-Mad2

Plasmid

#114021

Purpose

Lentiviral delivery of tagRFP-MAD2 expressed from the CMV promoter

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

tagRFP-Mad2 (MAD2L1) (MAD2L1 Human)

Use

Lentiviral

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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myc-hAtg13

Plasmid

#31965

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
3410 pBM272 Bcl-XL

Plasmid

#8772

Depositor

Stanley Korsmeyer

Article

Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Insert

Bcl-XL (Bcl2l1 Mouse)

Expression

Yeast

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pET28-T7-NlaCas3-NLS-6xHis

Plasmid

#180214

Purpose

Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas3c

Tags

6xHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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MAC3-EGFR

Plasmid

#222610

Purpose

Expression of MAC3-tagged EGFR

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFR (EGFR Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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sh-RNA hAtg13

Plasmid

#31971

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-ChRger3-TS-EYFP

Plasmid

#127240

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger3-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

hSyn1

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pQStrep2-PREI3

Plasmid

#31592

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

preimplantation protein 3 (MOB4 Human)

Tags

His and StrepII-tag

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-CaNAR2

Plasmid

#64730

Purpose

Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

Lyn-CaNAR2

Tags

Cerulean3, N-terminal targeting sequence from Lyn…

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

Plasmid

#182353

Purpose

GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-myc3-FBW5

Plasmid

#19905

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

F-box and WD repeat domain containing 5 (FBXW5 Human)

Tags

myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-PmCDA1

Plasmid

#79615

Purpose

Expresses dCas9-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

PmCDA1

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMRE147

Plasmid

#118499

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEY41

Plasmid

#191045

Purpose

dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

dat-1 (dat-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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