We narrowed to 69,214 results for: cloning

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pgRNAtet-lvaA

Plasmid

#106397

Purpose

Guide RNA plasmid targeting lvaA on BBR1-UP origin

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

sgRNA towards lvaA in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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AsCpf1-2NLS

Plasmid

#102565

Purpose

bacterial expression of AsCpf1-2NLS

Depositor

Jennifer Doudna

Publication

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

AsCpf1

Tags

6xHis-MBP-TEV and HA-2xSV40NLS

Expression

Bacterial

Promoter

T7

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET-MBP-NLS-Geo_st

Plasmid

#87703

Purpose

Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

Depositor

Jennifer Doudna

Insert

GeoCas9

Tags

10xHis-MBP-TEV

Expression

Bacterial

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLJM1-EGFP-BarcodeV5

Plasmid

#78629

Purpose

GESTALT V5 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Publication

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V5

Use

Lentiviral

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB104

Plasmid

#68558

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

GUS

Expression

Plant

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMJ923

Plasmid

#78313

Purpose

Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

Depositor

Martin Jinek

Publication

Burger et al Development. 2016 Apr 29. pii: dev.13480

Insert

SpCas9

Tags

HA-2xNLS-mCherry-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

human codon-optimized gene sequence

Promoter

T7

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV/3' Box_(GLuc)_TOP1

Plasmid

#68434

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSP977

Plasmid

#65774

Purpose

Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135E mutation in Cas9

Promoter

CMV & T7

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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TRPM2_pCSdest

Plasmid

#53920

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TRPM2 (TRPM2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pUC57 (Gent)

Plasmid

#54338

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMH3

Plasmid

#52528

Purpose

serves as PCR-template to generate HR donors for C-terminal GFP-tag

Depositor

Klaus Foerstemann

Publication

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

eGFP and Blasticigin resistance cassette

Use

Blunt cloning kit

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBABEpuro-ARAF

Plasmid

#51076

Purpose

mammalian expression of wt ARAF

Depositor

Matthew Meyerson

Publication

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOTTC374 - pAAV EF1a DIO iRFP

Plasmid

#47626

Purpose

An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Publication

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Infrared fluorescent protein

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MAP2-GCaMP3

Plasmid

#43917

Depositor

John Gearhart

Publication

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

GCaMP3

Use

Lentiviral

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

E247V in GCaMP3

Promoter

Human MAP2 promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti4/V5-DEST-P85

Plasmid

#40219

Depositor

Lynda Chin

Insert

P85 alpha (PIK3R1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pLenti4/V5-DEST-P85

Plasmid

#40219

Depositor

Lynda Chin

Insert

P85 alpha (PIK3R1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
MSCV-N GFP

Plasmid

#37855

Depositor

Karl Munger

Publication

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

GFP

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
MSCV-N GFP

Plasmid

#37855

Depositor

Karl Munger

Publication

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

GFP

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pENTR-L1-ESYN-R5

Plasmid

#32581

Depositor

Matthew Nolan

Publication

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Enhanced Synapsin Promoter

Use

Gateway Cloning

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pENTR-L1-ESYN-R5

Plasmid

#32581

Depositor

Matthew Nolan

Publication

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Enhanced Synapsin Promoter

Use

Gateway Cloning

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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