167,318 results

1. pcDNA3 Flag TSC2 (S939A/T1462A)

   Plasmid

   #14131

   Depositor

   Brendan Manning

   Article

   Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

   Insert

   TSC2 S939A/T1462A (TSC2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S939A/T1462A

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pLenti-CMV-MARCer-Blast

   Plasmid

   #160550

   Purpose

   labeling hypoxic cells

   Depositor

   Marc Vooijs

   Article

   Vermeer et al Dis Model Mech. 2020 Jul 30;13(7). pii:

   Insert

   MARCer (HIF1A Human)
   

   Use

   Lentiviral

   Tags

   eGFP-Cre

   Promoter

   CMV

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28C-mCherry-MED1LCD

   Plasmid

   #204404

   Purpose

   E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   MED1(947-1581) (MED1 Human)
   

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS SK mCherryROSAegfp

   Plasmid

   #54322

   Purpose

   Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

   Depositor

   Silvestro Conticello

   Article

   Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

   Insert

   mCherry and EGFP
   

   Use

   CRISPR, Mouse Targeting, and TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. CLK1

   Plasmid

   #155458

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   CLK1 (CLK1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 0_T7-mScarlet3

   Plasmid

   #225927

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223-PGK1

   Plasmid

   #23427

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PGK1 (PGK1 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-Nanoluc-ccdB

   Plasmid

   #87070

   Purpose

   Mammalian Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Tags

   Nanoluc luciferase

   Expression

   Mammalian

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b T7pCONS TIR-2 sfGFP

   Plasmid

   #154468

   Purpose

   pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Tags

   His-thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL39

    Plasmid

    #15458

    Depositor

    Chia Lee

    Article

    Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

    Insert

    
    

    Use

    S. aureus-e. coli shuttle vector

    Available Since

    Oct. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pscAAV-CAG-GFP

    Plasmid

    #83279

    Purpose

    recombinant AAV vector packaging self-complementary GFP under the CAG promoter

    Depositor

    Mark Kay

    Article

    Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

    Insert

    enhanced green fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C3-Sec3

    Plasmid

    #53755

    Purpose

    pEGFP-C3 plasmid that expresses Sec3

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Sec3 (EXOC1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pMSCVsin-PGK-GFP

    Plasmid

    #35615

    Depositor

    Patrick Salmon

    Insert

    GFP
    

    Use

    Retroviral

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCRISPRa-v2, Gene Expression (m5), top 5 sgRNAs/gene

    Pooled Library

    #84002

    Purpose

    Mouse CRISPRa Pooled Library targeting gene expression

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pMDJ39 - Peft-3 Cre (PATCs)

    Plasmid

    #191381

    Purpose

    High efficiency CRE recombinase expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Peft-3 Cre tbb-2 UTR
    

    Expression

    Worm

    Promoter

    Peft-3

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-lyso-pHluorin

    Plasmid

    #70113

    Purpose

    Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHluorin (Cd63 Mouse, Aequorea victoria)
    

    Tags

    HA-Tag and pHluorin

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496)

    Plasmid

    #214858

    Purpose

    Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    SYFP2
    

    Use

    AAV

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-Streptavidin-Glutamate_Tag

    Plasmid

    #46367

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Glutamate_Tag
    

    Tags

    6 glutamate tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

19. SadCas9 KRAB

    Plasmid

    #188504

    Purpose

    Expresses FLAG tagged SadCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. LENTICRISPR-UPP1_sgRNA1

    Plasmid

    #201634

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UPP1 (UPP1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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