164,794 results

1. pETM6-G6-vioABE-4A6-vioC

   Plasmid

   #73439

   Purpose

   Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE-P4A6-vioC
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PG6 and P4A6 (orthogonal T7-lac variants)

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. SaABE8e(TadA V106W)

   Plasmid

   #138509

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e V106W)-nSaCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   TadA-8e V106W

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191796

   Purpose

   vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13X
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   R84A, H89A, R739A, R740A, H745A, H746A, H747A

   Promoter

   CBh, SV40, hU6

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTC223

   Plasmid

   #70019

   Purpose

   Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7

   Depositor

   Daniel Voytas

   Article

   Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

   Insert

   Nuclease (Cas9/sgRNA) + Donor + GVR
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. GG-EBNA-OSK2M2L1-PP

   Plasmid

   #102902

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OSK2M2L1-gRNAs-PGK-puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-TACC3-His6

   Plasmid

   #69106

   Purpose

   For bacterial expression of recombinant human TACC3 tagged with GST and His.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   

   Use

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

   Plasmid

   #154473

   Purpose

   Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   SFFV

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCold-TF-hPLEKHM1

   Plasmid

   #64146

   Purpose

   For protein expression in E.coli

   Depositor

   Tamotsu Yoshimori

   Article

   Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

   Insert

   PLEKHM1 (PLEKHM1 Human)
   

   Use

   Tags

   6xHis-tag and Trigger Factor (TF)

   Expression

   Bacterial

   Mutation

   Promoter

   cspA

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-PTRE-tight-hM3Dq-mCherry

   Plasmid

   #66795

   Purpose

   Expresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTaging

   Depositor

   William Wisden

   Article

   Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

   Insert

   pTIGHT promoter-hM3Dq-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTIGHT TRE

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.GFAP.Cre.WPRE.hGH (AAV5)

    Viral Prep

    #105550-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV.GFAP.Cre.WPRE.hGH (#105550). In addition to the viral particles, you will also receive purified pAAV.GFAP.Cre.WPRE.hGH plasmid DNA. GFAP-driven Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    GFAP

    Tags

    None

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pMF-FU_TetO_GW_PPARG2

    Plasmid

    #193289

    Purpose

    Lentiviral construct that expresses a dox-inducible human PPARG2

    Depositor

    Rudolf Jaenisch

    Article

    Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

    Insert

    PPARG2 (PPARG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTrex-n-eGFP

    Plasmid

    #62544

    Purpose

    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome

    Depositor

    Rick Tarleton

    Article

    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

    Insert

    eGFP
    

    Use

    (1)trypanosoma cruzi expression of egfp (2) genom…

    Tags

    None

    Expression

    Mutation

    None

    Promoter

    Ribo-HX1

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPCX-VEcadTL

    Plasmid

    #45849

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    VE-cadherin (Cdh5 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. LeGO-mOrange2

    Plasmid

    #85212

    Purpose

    Lentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    mOrange2
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)

    Plasmid

    #86416

    Purpose

    ONM/ER split GFP11 reporter with mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-GFP11-mCherry-Scs2TM
    

    Use

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    NOP1

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEarleyGate 100RMOA mPing

    Plasmid

    #196137

    Purpose

    Measures mPing transposition in plants

    Depositor

    Charles Nathan Hancock

    Article

    Renken et al MicroPubl Biol. 2023 May 16;2023:10.1791

    Inserts

    Pong TPase LA T2A ORF1SC1 ONE
    mPing
    

    Use

    Binary

    Tags

    Expression

    Plant

    Mutation

    TPase NES sequence mutated L418A, L420A; T2A pept…

    Promoter

    35S and RPS5a

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TK-GFP-Nluc-P2A-neo-TK

    Plasmid

    #134896

    Purpose

    Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Insert

    TK-GFP-Nluc-P2A-Neo-TK
    

    Use

    Cryptosporidium expression

    Tags

    Expression

    Mutation

    Promoter

    Cryptosporidium actin and enolase

    Available since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAnc80L65AAP

    Plasmid

    #92307

    Purpose

    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

    Depositor

    Luk Vandenberghe

    Article

    Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

    Insert

    Ancestral AAV Capsid Anc80L65AAP
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Rep2

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGT-Ah1

    Plasmid

    #122568

    Purpose

    MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    beta-lactamase/sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21-pelB-LaG94-5

    Plasmid

    #228965

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-5
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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