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172,720 results

Showing: 5121 - 5140 of 172720 results

  1. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available Since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

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  5. pG2K

    Plasmid
    #71742
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71742-plasmid-map-sequence-id-130147

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  8. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid
    #229676
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Halo, 3xALFA
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229676-plasmid-map-sequence-id-457076

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  11. pJAT31

    Plasmid
    #204291
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204291-plasmid-map-sequence-id-405841

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  16. FUW-tetO-EGFP

    Plasmid
    #84041
    Purpose
    3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter
    Depositor
    Stefano Piccolo
    Article
    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    TRE-CMV
    Available Since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    84041-plasmid-map-sequence-id-490455

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  19. WT Smarca4-V5 IRES-puro

    Plasmid
    #107058
    Purpose
    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107058-plasmid-map-sequence-id-201960

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Showing: 5121 - 5140 of 172720 results