We narrowed to 13,844 results for: cas9 genes

1. pCMV-T7-TadCBEd-SpG-P2A-EGFP (BKS777/AHK571)

   Plasmid

   #223124

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd-SpG with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadCBEd-SpG-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpG=D10A/D1135L/S1136W/G1218…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGmCherry-gRNA

   Plasmid

   #87110

   Purpose

   gRNA cloning vector with CAGmCherry

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

   Plasmid

   #112677

   Purpose

   An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Nuclear-localized floxed-mCherry EGFP
   

   Use

   AAV

   Tags

   Nuclear localization signal

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

   Plasmid

   #113158

   Purpose

   An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   gRNA for rat TH
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

   Plasmid

   #113156

   Purpose

   An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat Rosa26
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

   Plasmid

   #113159

   Purpose

   An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   gRNA for rat TH
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

   Plasmid

   #113155

   Purpose

   An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat TH
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

   Plasmid

   #113157

   Purpose

   An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat MANF
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. DDR_MKOv4

   Pooled Library

   #140219

   Purpose

   CRISPR DNA damage response library

   Depositor

   Junjie Chen

   Article

   Su et al DNA Repair (Amst). 2020 Jan 16;87:102803

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pFOXA3.1.0-gDNA

    Plasmid

    #112417

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXA3

    Depositor

    Kevin White

    Insert

    FOXA3 (FOXA3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNR2F1.1.0-gDNA

    Plasmid

    #112484

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR2F1

    Depositor

    Kevin White

    Insert

    NR2F1 (NR2F1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pELF4.1.0-gDNA

    Plasmid

    #112467

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELF4

    Depositor

    Kevin White

    Insert

    ELF4 (ELF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMYT095

    Plasmid

    #198866

    Purpose

    CRISPR/Cas9 Vector with URA3 selectable marker to be used in the Ellis MYT system

    Depositor

    Tom Ellis

    Article

    Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRVV651

    Plasmid

    #87631

    Purpose

    Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1 attP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMYT096

    Plasmid

    #198867

    Purpose

    Transient CRISPR/Cas9 Vector without a selectable marker to be used in the Ellis MYT system

    Depositor

    Tom Ellis

    Article

    Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRVV647

    Plasmid

    #87630

    Purpose

    Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1 attP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

    Plasmid

    #218557

    Purpose

    Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

    Depositor

    Alejandro Chavez

    Insert

    MCP-HSF1-VIRF2
    

    Use

    Lentiviral

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    Available since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRVV653

    Plasmid

    #89474

    Purpose

    Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1 attP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRVV598

    Plasmid

    #87629

    Purpose

    Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1 attP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pV1025

    Plasmid

    #111424

    Purpose

    Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAVS1-DRGFP

    Plasmid

    #113193

    Purpose

    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    DR-GFP
    

    Use

    Donor construct with t2a-hygromycin for gene trap…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR221_SMAD2_HAe2_Bsd

    Plasmid

    #167166

    Purpose

    Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.

    Depositor

    Alexander Loewer

    Insert

    Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
    

    Use

    Repair template for gene editing in human cells

    Tags

    blasticidin

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid

    #99698

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV

    Depositor

    George Church

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pATF4.1.0-gDNA

    Plasmid

    #113772

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4

    Depositor

    Kevin White

    Insert

    ATF4 (ATF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJUNB.1.0-gDNA

    Plasmid

    #112409

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor JUNB

    Depositor

    Kevin White

    Insert

    JUNB (JUNB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pELK3.1.0-gDNA

    Plasmid

    #112418

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3

    Depositor

    Kevin White

    Insert

    ELK3 (ELK3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJW1259

    Plasmid

    #61251

    Purpose

    Cas9 plasmid with sgRNA deleted; for use in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    Deletion
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Deleted sgRNA(F+E) sequence from pJW1219

    Promoter

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pESR1.1.0-gDNA

    Plasmid

    #112433

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1

    Depositor

    Kevin White

    Insert

    ESR1 (ESR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTFE3.1.0-gDNA

    Plasmid

    #112473

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFE3

    Depositor

    Kevin White

    Insert

    TFE3 (TFE3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pERF.1.0-gDNA

    Plasmid

    #112395

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ERF

    Depositor

    Kevin White

    Insert

    ERF (ERF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBCL6.1.0-gDNA

    Plasmid

    #112485

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor BCL6

    Depositor

    Kevin White

    Insert

    BCL6 (BCL6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Construct 3 - U6p_20nt1_mod2_sgRNA (stgRNA)

    Plasmid

    #81246

    Purpose

    For assaying self-targeting activity via Cas9

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    sgRNA
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pE2F3.3.1-gDNA

    Plasmid

    #113776

    Purpose

    CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor E2F3

    Depositor

    Kevin White

    Insert

    E2F3 (E2F3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Construct 1 - U6p_20nt1_WT_sgRNA

    Plasmid

    #81245

    Purpose

    For assaying self-targeting activity via Cas9

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    sgRNA
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCREB1.1.0-gDNA

    Plasmid

    #113770

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CREB1

    Depositor

    Kevin White

    Insert

    CREB1 (CREB1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCSDC2.1.0-gDNA

    Plasmid

    #113781

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CSDC2

    Depositor

    Kevin White

    Insert

    CSDC2 (CSDC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCLOCK.1.0-gDNA

    Plasmid

    #112406

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CLOCK

    Depositor

    Kevin White

    Insert

    CLOCK (CLOCK Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFOXO4.1.0-gDNA

    Plasmid

    #112408

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXO4

    Depositor

    Kevin White

    Insert

    FOXO4 (FOXO4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pX458-MAFB-sg

    Plasmid

    #194720

    Purpose

    px458 with guide RNA that target hMAFB

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    Cas9 (MAFB Streptococcus pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRBPJ.1.0-gDNA

    Plasmid

    #132474

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RBPJ (RBPJ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge