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pAAV-EF1a-Cre

Plasmid

#55636

Purpose

Expresses Cre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV11

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Ef1a

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon DREADD Gi-mCherry

Plasmid

#177669

Purpose

Expresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cells

Depositor

Karl Deisseroth

Article

Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

Insert

DREADD Gi-mCherry

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-BEC enhancer-minBG-iCre(R279T)-4x6T

Plasmid

#236727

Purpose

Brain Endothelial Cell Enhancer with 4x6T miRNA targeting cassette For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.

Depositor

Xiangmin Xu

Article

Velazquez-Rivera et al Neuron. 2025 May 21;113(10):1562-1578.e6

Inserts

cis-regulatory elements 89763
iCre(R297T)
4x6T miRNA targeting cassette

Use

AAV

Mutation

R297T

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flpo

Plasmid

#55637

Purpose

Expresses Flpo in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde, AAV1, and AAV2

Insert

Flpo

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC476 - pAAV c-fos eYFP

Plasmid

#47907

Purpose

An AAV vector that expresses eYFP under the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

enhanced Yellow Fluorescent Protein

Use

AAV

Promoter

c-fos

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV2-TBG-FLAG-mRIPK1

Plasmid

#115341

Purpose

Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1

Depositor

Junying Yuan

Article

Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

Insert

RIPK1 (Ripk1 Mouse)

Use

AAV

Tags

Flag

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-Cre

Plasmid

#196140

Purpose

AAV-mediated expression of Cre under the CAG promoter .

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Cre

Use

AAV

Expression

Mammalian

Mutation

codon diversified

Promoter

CAG

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Chronos-GFP

Plasmid

#59170

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CRE-CytoTape-V5

Plasmid

#239430

Purpose

CytoTape signal monomer for recording phosphorylated CREB protein activity

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-V5

Use

AAV

Tags

V5-dMBP

Expression

Mammalian

Promoter

CRE promoter

Available Since

Nov. 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Cbh_v5 AAV-CBE C-terminal

Plasmid

#137176

Purpose

AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

Cbh

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-rtTA3G-TetOn3G

Plasmid

#250672

Purpose

Drive Tet-ON activator expression from human synapsin promoter for doxycycline-inducible transgene activation

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

rtTA3G-TetOn3G

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-Ucp1-Cre-miR122

Plasmid

#228408

Purpose

Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

Depositor

Joerg Heeren

Article

Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

Inserts

cre recombinase with SV40 NLS
Cre recombinase with SV40 NLS

Use

AAV

Promoter

rat Ucp1 enhancer-promoter

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-sRGECO

Plasmid

#137128

Purpose

Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Ef1a-DIO-BiPOLES-mCerulean

Plasmid

#154949

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mScarlet

Plasmid

#183461

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mScarlet

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

Plasmid

#207863

Purpose

AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-mCherry

Plasmid

#137134

Purpose

Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

Plasmid

#101060

Purpose

AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Plasmid

#207861

Purpose

AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_5-HT1.0

Plasmid

#140552

Purpose

Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons

Depositor

Yulong Li

Article

Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

Has Service

AAV9

Insert

5-HT sensor GRAB_5-HT1.0

Use

AAV

Promoter

hSyn

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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