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pRK5_tAKARα-T391A

Plasmid

#119920

Purpose

Mammalian expression of the tAKARα sensor with T391A mutation

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARα-T391A

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a H759A

Plasmid

#113518

Purpose

Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Mutation

H759A

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCS-PluxIlux

Plasmid

#47641

Purpose

wt luxI promoter in pCS26

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

PluxI

Use

Synthetic Biology

Expression

Bacterial

Promoter

PluxI

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pBT271.359

Plasmid

#127845

Purpose

clone A3A CBEs by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

A3A

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-XIAP1-351

Plasmid

#8342

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP1-351 (XIAP Human)

Tags

GST

Expression

Bacterial

Mutation

deleted amino acids 352-496

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
RS314-H

Plasmid

#17541

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

Gal1-p53 (TP53 Human)

Expression

Yeast

Mutation

R273H

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pFastBac GST-SAS6

Plasmid

#80273

Purpose

baculovirus creation plasmid for GST-SAS6

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

SAS6 (SASS6 Human)

Tags

GST

Expression

Insect

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-mCherry

Plasmid

#67162

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

mCherry

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
mCherry-uniRapR-P-Vav2(DPZ)

Plasmid

#91875

Purpose

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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RS314-SN

Plasmid

#17539

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

Gal1-p53 (TP53 Human)

Expression

Yeast

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pBT138b-pro1

Plasmid

#138513

Purpose

P3h

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL + STS

Plasmid

#139790

Purpose

expression of biosynthetic enzymes 4CL and STS

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL
STS

Expression

Bacterial

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pARv-RFP

Plasmid

#60021

Purpose

pARv-RFP is a fluorescent reporter plasmid, that allows fast and easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the transfected cells.

Depositor

Radislav Sedláček

Article

Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pSV2-c-erbB2

Plasmid

#16250

Depositor

Mien-Chie Hung

Article

Kwong et al Mol Carcinog. 1998 Oct . 23(2):62-8.

Insert

c-erbB2 (ERBB2 Human)

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
SyGCaMP5G

Plasmid

#162519

Purpose

Synaptically targeted GCaMP5G

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GCaMP5G

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAG372-mito-greenFAST

Plasmid

#160357

Purpose

Expresses mito-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

mito-greenFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET47b-T7-dAsCas12f

Plasmid

#204641

Purpose

Expression of an inactive AsCas12f in E. coli

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

inactive AsCas12f

Tags

6xHis

Expression

Bacterial

Mutation

D225A

Promoter

T7

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCHNFI-B2

Plasmid

#31405

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

Insert

Nuclear Factor IB isoform 2 (Nfib Mouse)

Tags

HA

Expression

Mammalian

Mutation

Not the same as reference seq. See sequence for m…

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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hNedd4-2 delta C2

Plasmid

#83434

Purpose

Nedd4L-deltaC2 with His Xpress tag

Depositor

Christie Thomas

Article

Itani et al Am J Physiol Renal Physiol. 2005 Aug;289

Insert

human Nedd4L without C2 domain (NEDD4L Human)

Tags

His Tag (6x) and Xpress Epitope Tag

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pC0.053

Plasmid

#119571

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

PnirA

Use

Synthetic Biology

Mutation

RBS* added (22 bp) upstream of ATG.

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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