We narrowed to 10,073 results for: control

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pCALNL-mutKir2.1

Plasmid

#125579

Purpose

Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

A non-conducting mutant (GYG -> AAA).

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1+13

Plasmid

#61087

Purpose

Express N-terminal GST tagged-mouse BIN1+13 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 13, excluding exon 7, 11, 14-17

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pDest27-mBIN1

Plasmid

#61086

Purpose

Express N-terminal GST tagged-mouse BIN1 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

smallest constitutive BIN1excluding exon 7, 11, 13-17

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
KAT6B-luciferase

Plasmid

#113353

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

KAT6B-enhancer (KAT6B Human)

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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SHIP2 WT pBABE-His-Xpress

Plasmid

#126584

Purpose

Retroviral infection of MEF cells (puromycin selection)

Depositor

Christophe Erneux

Article

Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. d

Insert

SHIP2 (INPPL1 Human)

Use

Retroviral

Tags

His Xpress (N-terminal)

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINTphiC31

Plasmid

#127535

Purpose

Plasmid encodes A. thaliana codon optimized Integrase phiC31.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase phiC31 coding sequence codon optimized for A. thaliana expression.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-CDC34

Plasmid

#52731

Purpose

retroviral expression of human CDC34

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

CDC34 (CDC34 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

Plasmid

#205098

Purpose

Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Björefeldt et al Neurophotonics. 2024 Apr;11(2):021005. d

Insert

mNeonGreen, eKL9h, and Volvox channelrhodopsin-1

Use

AAV

Promoter

hSyn

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro)

Plasmid

#174634

Purpose

Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVDfast-mCherry-SSPB(micro) (Kif1a Synthetic, Mouse)

Tags

mCherry-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

Promoter

Chicken beta-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV gfaABC1D 2xLyn-ERex-Venus-bPAC(F198Y)

Plasmid

#171117

Purpose

Astrocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus-bPAC(F198Y)

Use

AAV

Tags

Venus

Expression

Mammalian

Promoter

gfaABC1D

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry-FKBP-GEF(ITSN1)

Plasmid

#222631

Purpose

Cdc42 activation using rapamycin

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

mCherry-FKBP-GEF(ITSN1) (ITSN1 Human)

Tags

mCherry-FKBP

Expression

Mammalian

Promoter

CAG

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-NLC-DHFR-SpCas9

Plasmid

#124523

Purpose

Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

Depositor

Amit Choudhary

Article

Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

Insert

NLC-DHFR-SpCas9

Use

AAV

Tags

Destabilized domain of E.coli dihydrofolate reduc…

Expression

Mammalian

Promoter

Cbh

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3 4KR

Plasmid

#78813

Purpose

To overexpress RIPK3 4KR in Mammalian Cell

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Mutation

K55R, K89R, K363R, K501R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINT13

Plasmid

#127534

Purpose

Plasmid encodes A. thaliana codon optimized Integrase 13.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 13 coding sequence codon optimized for A. thaliana expression.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-Zfp423-deltaSBD

Plasmid

#35971

Depositor

Bruce Spiegelman

Article

Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

Insert

Zinc Finger Protein 423 (Zfp423 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

lacks zinc fingers 14-19 (aa 640 -838) (delta SMA…

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-delta6xNHL-LIN-41

Plasmid

#52722

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 593–868

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-RIPK1 3KR

Plasmid

#78847

Purpose

To detect localization of RIPK1 3KR protein in live mammalian cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

K571R, K604R, K627R

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
CRY2clust-mCherry-RANK-P2A-CIBNcaax

Plasmid

#208613

Purpose

Mammalian expression of CRY2clust-mCherry-RANK-P2A-CIBNcaax (Opto-RANKm)

Depositor

Tomohiro Ishii, Takao Nakata

Article

Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

Insert

CRY2clust-mCherry-RANK-P2A-CIBNcaax (Cry2 Mustard Weed, Mouse)

Tags

CAAX and mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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LightR-bRaf-mVenus

Plasmid

#162154

Purpose

Expresses LightR-bRaf-mVenus in mammalian cells

Depositor

Andrei Karginov

Article

Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

Insert

LightR-bRaf-mVenus

Tags

mVenus

Expression

Mammalian

Mutation

Valine 600 is substituted with Glutamic acid

Promoter

CMV

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMT[Hygro]_GBP-3xFlag-DEST

Plasmid

#157813

Purpose

A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection

Depositor

Zoltan Lipinszki

Article

Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:

Insert

GFP-binding protein

Use

For drosophila cells

Tags

3xFlag

Expression

Insect

Promoter

metallothionein

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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