We narrowed to 41,904 results for: tro

1. pX330.sgNf1.4

   Plasmid

   #92028

   Purpose

   pX330 sgNf1.4 for Nf1 deletion

   Depositor

   Wen Xue

   Article

   Song et al Gastroenterology. 2017 Apr;152(5):1161-1

   Insert

   sgNf1.4
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

2. PXL

   Plasmid

   #75349

   Purpose

   PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-sgRNA-GFP

   Plasmid

   #200068

   Purpose

   For the knock down of GFP with an astrocyte specific mCherry expression

   Depositor

   Baljit Khakh

   Article

   Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

   Insert

   U6-sgRNA-GFP
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   GfaABC1D-mCherry

   Expression

   Mutation

   Promoter

   U6, GfaABC1D

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-IGK-dAPEX2-KDEL

   Plasmid

   #117182

   Purpose

   Plasmid name in publication: pAAV-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   IGK-dAPEX2-KDEL
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xFLAG-dCas9/pMXs-neo

   Plasmid

   #51260

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-CRY2-5ptaseOCRL(D523G)

   Plasmid

   #66837

   Purpose

   Expression of inositol 5-phosphatase domain of OCRL(D523G - catalytically inactive mutant). Binds to CIBN upon blue light illumination.

   Depositor

   Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   5ptaseOCRL (OCRL Human)
   

   Use

   Tags

   mCherry-CRY2

   Expression

   Mammalian

   Mutation

   D523G

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBMN-2xFKBP-mEGFP-NDP52

   Plasmid

   #110465

   Purpose

   retrovirus construct for stable expressing mEGFP tagged NDP52

   Depositor

   Richard Youle

   Article

   Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

   Insert

   NDP52 (CALCOCO2 Human)
   

   Use

   Retroviral; Retrovirus

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LiPOP1-NanoLuc

   Plasmid

   #168265

   Purpose

   Chemo-optogenetic control of necroptotic cell death by coupling LiPOP1 with NanoLuc-aided bioluminescence optogenetic stimulation (NanoLOGS)

   Depositor

   Yubin Zhou

   Article

   He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

   Insert

   MLKL(1-125) (MLKL Human)
   

   Use

   Tags

   mCherry, NanoLuc, CRY2-PHR

   Expression

   Mammalian

   Mutation

   H15A, K16A, R17A

   Promoter

   CMV

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVUTHshGATA1-tTR-KRAB

   Plasmid

   #11650

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. E. coli expression: Z-lock cofilin dark state mutant

    Plasmid

    #141135

    Purpose

    a control for optogenetic cofilin analog

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D3 deletion, I32F in Zdk2

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mKate2-P2A-APEX2-ML1N*2

    Plasmid

    #67665

    Purpose

    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-ML1N*2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJS145

    Plasmid

    #179159

    Purpose

    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

    Depositor

    Iva Greenwald

    Article

    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

    Insert

    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Flexon replacing 2nd intron of 2xNLS::GFP

    Promoter

    rps-27p

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV2/9-CW3SL-mCherry-DrTrkB

    Plasmid

    #184002

    Purpose

    AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

    Depositor

    Dmitry Gerashchenko, Vladislav Verkhusha

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrTrkB (NTRK2 Human)
    

    Use

    AAV

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tol2-LyzC-Cas9-pA-Cry-GFP

    Plasmid

    #168239

    Purpose

    "neutrophil specific cas9 expression in zebrafish with green lens"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Cas9
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX 4T1 ERbeta

    Plasmid

    #35563

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    Estrogen Receptor beta (ESR2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. cyclin D1-HA-pBabe-Hygro

    Plasmid

    #22703

    Depositor

    William Kaelin

    Article

    Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

    Insert

    cyclin D1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. CRY2-OCRL(D523G)

    Plasmid

    #79567

    Purpose

    expression of CRY2PHR fused to phosphatase dead mutant of OCRL

    Depositor

    Olof Idevall-Hagren, Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    OCRL (OCRL Human)
    

    Use

    Tags

    CRY2 (photolyase homology region domain of Arabid…

    Expression

    Mammalian

    Mutation

    aa 234_539 and phosphatase mutation, D523G

    Promoter

    CMV

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet

    Plasmid

    #130708

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet
    

    Use

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CIBN-GFP-TMD

    Plasmid

    #79571

    Purpose

    expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)

    Depositor

    Olof Idevall-Hagren, Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    syntaxin 1A (Stx1a Mouse)
    

    Use

    Tags

    CIBN and EGFP

    Expression

    Mammalian

    Mutation

    aa 249_288 (C-terminal region (polybasic stretch …

    Promoter

    CMV

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3xFLAG-dCas9/pMSCVneo

    Plasmid

    #134982

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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