We narrowed to 152,943 results for: DES

1. pBEG R2-eCFP-L3 #BGV202

   Plasmid

   #48977

   Purpose

   Supplies the selection marker (eCFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   eCFP
   

   Use

   Gateway cloning vector

   Tags

   IRES (strong)

   Expression

   Mutation

   Promoter

   NA

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pDRF’- AmTryoshka1;3-GS

   Plasmid

   #100967

   Purpose

   Yeast expression of control vector for fluorescent sensors reporting the activity of ammonium transceptors.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   AmTryoshka1;3-GS (AMT1;3 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PMA

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYZ_NAND2_L17_S19_S11

   Plasmid

   #132730

   Purpose

   T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11

   Depositor

   Alexander Green, Peng Yin

   Article

   Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

   Insert

   3WJ_NAND2_L17_S19_S11
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. ERAS

   Plasmid

   #55656

   Purpose

   expression of mouse ERAS

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   ERAS (Eras Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

5. GEM

   Plasmid

   #55680

   Purpose

   expression of mouse GEM

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   GEM (Gem Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCA24N-ligase-pprA

   Plasmid

   #87744

   Purpose

   IPTG-inducible expression of ligase-pprA fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and codon optimized pprA (from Deinococcus …

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAN1935

   Plasmid

   #127203

   Purpose

   Constitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchC

   Depositor

   Hana El-Samad

   Article

   Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

   Insert

   pTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET a sfMatryoshCaMP6s

   Plasmid

   #100020

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   sfMatryoshCaMP6s
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   cpEGFP replaced with superfolder cpGFP

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAN1232

   Plasmid

   #127164

   Purpose

   Vector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)

   Depositor

   Hana El-Samad

   Article

   Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586

   Insert

   pRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ABE8e(TadA-8e V82G)

    Plasmid

    #138494

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V82G)-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TadA-8e V82G

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. (PM-S608E)FLAG-Cep215-FL

    Plasmid

    #106906

    Purpose

    Expresses murine phospho-mimetic CEP215 at S608

    Depositor

    Heidi Hehnly

    Article

    Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

    Insert

    CEP215 (Cdk5rap2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine-608 to Glutamic Acid

    Promoter

    CMV

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-h-mmPtch1-B-HA

    Plasmid

    #120902

    Purpose

    Expresses Ptch1-B for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAN1572

    Plasmid

    #127202

    Purpose

    Dual inducible expression of Venus-degronSwitchA and KeyA-BFP

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    pGal1-Venus-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXPR_BRD003 sgLacZ-1

    Plasmid

    #74968

    Purpose

    sgLacZ-1, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    LacZ
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXPR_BRD003 sgLacZ-2

    Plasmid

    #74969

    Purpose

    sgLacZ-2, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    LacZ
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCLX-MIFE-hM3Dq-PGK-mCherry

    Plasmid

    #114294

    Purpose

    Mifepristone-inducible expression of hM3Dq protein

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    hM3Dq
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CSII-IDR2-chimpTRIM5-FOS

    Plasmid

    #79065

    Purpose

    Express chimpanzee TRIM5alpha in mammalan cells

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    TRIM5alpha(chimpanzee)-FOS
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac1-Kozak-rhTRIM5alpha-PFOS

    Plasmid

    #79038

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    rhesus TRIM5alha-PFOS
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-Pchi-HMG

    Plasmid

    #231540

    Purpose

    Expresses the HMG of Pchi Sox with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Sox HMG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-Salhel-HMG

    Plasmid

    #231539

    Purpose

    Expresses the HMG of Salhel Sox-I with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Sox-I HMG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge