We narrowed to 33,582 results for: Eng
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Plasmid#79050PurposeExpress wild type AlkB protein in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
TagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-RhoBAST-MS2
Plasmid#196342PurposeOverexpression of circular RhoBAST-MS2DepositorInsertTornado-RhoBAST-MS2
ExpressionMammalianPromoterU6+27Available SinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-M
Plasmid#182280PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.DepositorInsertcAMPFIRE-M (cAMP sensor)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-UAS-Dendra2
Plasmid#97145PurposeFor Gal4/UAS expression systemDepositorInsertDendra2
UseZebrafishAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF.hHES1.Ubc.GFP
Plasmid#17624DepositorAvailable SinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT51
Plasmid#223423PurposeT-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.DepositorInsert2x35s-LbCas12a-RRV-2x35s-crRNA scaffold
UseCRISPRTagsNLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEZ-1
Plasmid#51868PurposeExpression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegansDepositorInsertpsdhb-1::mtLS-cpYFP
ExpressionWormPromotersdhb-1Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT39
Plasmid#223411PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants select.DepositorInsert2x35s-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
double cut NH-donor
Plasmid#83576PurposeeGFP reporter donor containing double SA cutting sites for NHEJ knock-inDepositorInserteGFP
UseNhej donorAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT48
Plasmid#223420PurposeT-DNA vector for Mb2Cas12a-RVRR based mutagenesis for dicot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.DepositorInsert2x35s-Mb2Cas12a-RVRR-2x35s-crRNA scaffold
UseCRISPRTagsNLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT42
Plasmid#223414PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sg-A
Plasmid#83807PurposeExpress sgRNA targeting SA siteDepositorInsertSgRNA
UseCRISPRAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC150-PBLHL-4xHS-EF1a-DEST
Plasmid#48234PurposePiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection markerDepositorTypeEmpty backboneUsePiggybacExpressionMammalianPromoterEF1aAvailable SinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCgCas9_recT
Plasmid#153337PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; KanrDepositorInsertCas9
UseCRISPRExpressionBacterialAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB-Tg
Plasmid#28220DepositorInsertSV40 large T
ExpressionMammalianAvailable SinceApril 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT37
Plasmid#223409PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.DepositorInsertAtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-Pepper-RhoBAST
Plasmid#196343PurposeOverexpression of circular Pepper-RhoBASTDepositorInsertTornado-Pepper-RhoBAST
ExpressionMammalianPromoterU6+27Available SinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
Plasmid#41863PurposeThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.DepositorArticleInsertU6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMSH2
Plasmid#129423PurposeExpression human MSH2 protein using baculovirusDepositorAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only