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pSCT152: pHelper(AjTn6022)_ΔTniQ

Plasmid

#200855

Purpose

Inducible expression of AjTn6022 proteins (except TniQ).

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT149: pHelper(AjTn6022)_ΔTnsA

Plasmid

#200852

Purpose

Inducible expression of AjTn6022 proteins (except TnsA).

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT150: pHelper(AjTn6022)_ΔTnsB

Plasmid

#200853

Purpose

Inducible expression of AjTn6022 proteins (except TnsB).

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT147: pHelper(AjTn6022)

Plasmid

#200851

Purpose

Inducible expression of AjTn6022 proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ103-rbs1-m1

Plasmid

#204463

Purpose

This is a biosensor with improved specificity to 3OC12-HSL, containing LasR A127N mutation.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

Inserts

quorum sensing regulator LasR (lasR )
eYFP

Use

Synthetic Biology

Mutation

LasR A127N mutation

Promoter

In an operon and PLas

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ103-rbs1-m2

Plasmid

#204464

Purpose

This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L128C mutation.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

Inserts

quorum sensing regulator LasR (lasR )
eYFP

Use

Synthetic Biology

Mutation

LasR L128C mutation

Promoter

In an operon and PLas

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pmirGLO-mir302-8mer4x (pEZ185)

Plasmid

#229059

Purpose

Dual-luciferase reporter containing 4 copies of the 8mer site of human miR-302a

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Insert

Human miR-302a binding sites

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCI5-CasRx-NLS-HA (pKW919)

Plasmid

#229047

Purpose

Mammalian expression of CasRx-NLS-HA

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Insert

CasRx (RfxCas13d)

Use

CRISPR

Tags

NLS and NLS-HA

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRS316-SSD1-L1374

Plasmid

#226278

Purpose

Plasmid expressing the SSD1 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SSD1 (SSD1 Budding Yeast)

Expression

Yeast

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET29_anti-SARS-CoV-2-NM1226

Plasmid

#209422

Purpose

Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1226

Tags

Myc-6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-NM1230

Plasmid

#209423

Purpose

Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1230

Tags

Myc-6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-Nanobody-C5

Plasmid

#209420

Purpose

Express anti-SARS-CoV-2-Nanobody C5 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-Nanobody-C5

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-Nanobody-F2

Plasmid

#209421

Purpose

Express anti-SARS-CoV-2-Nanobody F2 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-Nanobody-F2

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pRS315-SEC22-NCYC110

Plasmid

#226273

Purpose

Plasmid expressing the SEC22 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-L1374

Plasmid

#226271

Purpose

Plasmid expressing the SEC22 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-UWOPS872421

Plasmid

#226272

Purpose

Plasmid expressing the SEC22 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-S288C

Plasmid

#226270

Purpose

Plasmid expressing the SEC22 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pML107-PMR1

Plasmid

#226264

Purpose

Plasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

PMR1 gRNA (PMR1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SSD1-NCYC110

Plasmid

#226279

Purpose

Plasmid expressing the SSD1 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SSD1 (SSD1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDLterm

Plasmid

#211903

Purpose

Base plasmid for dual-luciferase constructs to measure terminator strength

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Type

Empty backbone

Use

Luciferase

Expression

Plant

Promoter

35S enhancer + 35S minimal promoter

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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