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EF1a_KLF1_P2A_Hygro_Barcode

Plasmid

#120488

Purpose

Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF1 (KLF1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYCL_P2A_Hygro_Barcode

Plasmid

#120462

Purpose

Barcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYCL (MYCL Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-HaloTag-Puromycin

Plasmid

#167208

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

HaloTag

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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HLA_DRA_01_01

Plasmid

#165012

Purpose

Expression of HLA-DRA*01:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRA (HLA-DRA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GEM2-Halo-FRB

Plasmid

#197056

Purpose

Expression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-mir124a

Plasmid

#61539

Purpose

Expresses mir124a in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

mir124a (MIR124-1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-TetOne-Puro-mitoEcSTH-FLAG

Plasmid

#218629

Purpose

This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

mitoEcSTH

Use

Lentiviral and Synthetic Biology

Tags

FLAG and MTS

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_HNF1A_P2A_Hygro_Barcode

Plasmid

#120448

Purpose

Barcoded lentiviral vector to express HNF1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HNF1A (HNF1A Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-IP3KA

Plasmid

#32529

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KA (ITPKA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
GST-N1IC

Plasmid

#47612

Purpose

bacterial expression of GST-myc-Notch1 IC

Depositor

Urban Lendahl

Article

Beatus et al Development. 1999 Sep;126(17):3925-35.

Insert

Notch1 intracellular domain (Notch1 Mouse)

Tags

GST and myc

Expression

Bacterial

Mutation

contains amino acids 1753-2531

Promoter

tac

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
TU#707

Plasmid

#15276

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzgfp

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pDSG320

Plasmid

#115597

Purpose

surface-displayed Nb2, used in composability experiments; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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G12-CASE

Plasmid

#190714

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

Depositor

Gunnar Schulte

Article

Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.

Inserts

GNB3 (GNB3 Human)
GNGT9
GNA12 (GNA12 Human)

Use

Luciferase

Tags

Nluc is inserted at A133/F134 within GNA12 and cp…

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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LB1366 (Bcl-2 promoter with MHS1234)

Plasmid

#15388

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with IgH full enhancer MHS1234

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pFH3.2_mTagBFP2-MS2x12

Plasmid

#205558

Purpose

Express mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mTagBFP2

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.MTRIA-OT

Plasmid

#184594

Purpose

For production of AAV for a fluorescent sensor for oxytocin

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

AAV

Promoter

Human synapsin promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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TOP-H2B-YFP-DD

Plasmid

#96891

Purpose

Reporter to monitor translation of 5'TOP mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

TOP-H2B-YFP-DD

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pWarf(+)

Plasmid

#34562

Depositor

Jeff Abramson

Article

Hsieh et al Protein Sci. 2010 Apr;19(4):868-80.

Insert

HRV 3C-GPA-GFP-8HIS

Expression

Bacterial

Available Since

Feb. 23, 2012

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-SART1

Plasmid

#38087

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

SART1 squamous cell carcinoma antigen recognized by T cells (SART1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-WASP-GBD

Plasmid

#30113

Depositor

David Sacks

Article

Kim et al J Biol Chem. 2000 Nov 24. 275(47):36999-

Insert

WASP-GBD (WAS, WAS Human)

Tags

GST

Expression

Bacterial

Mutation

Contains the Cdc42-interacting domain of WASP (aa…

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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