We narrowed to 7,267 results for: trac

1. pYPQ132A2.0

   Plasmid

   #99887

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVC-Ds-DrU6a:sgRNA-com-Ds

   Plasmid

   #119077

   Purpose

   To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:2xCom-sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish U6a promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVC-Ds-DrU6a:sgRNA-MS2-Ds

   Plasmid

   #119076

   Purpose

   To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:2xMS2-sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish U6a promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ133A2.0

   Plasmid

   #99891

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 RL3Sc

   Plasmid

   #109365

   Purpose

   Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCHOP10.pCDNA1

   Plasmid

   #21899

   Depositor

   David Ron

   Article

   Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

   Insert

   CHOP10 (Ddit3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pSW002-PpsbA-E2-Crimson

   Plasmid

   #111258

   Purpose

   Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with E2-Crimson

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   E2-Crimson
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PpsbA

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ133C2.0

   Plasmid

   #99893

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMRE160

   Plasmid

   #118508

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   TagBFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ131C2.0

    Plasmid

    #99886

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU6

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ132C2.0

    Plasmid

    #99889

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU6

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSMOX-mNG::LacI-V3

    Plasmid

    #210224

    Purpose

    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    mNeongreen Lac repressor fusion protein
    

    Use

    Trypanosoma brucei expression

    Tags

    Expression

    Mutation

    Promoter

    Pol II readthrough transcription after integration

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRJPaph-lacZYA

    Plasmid

    #67037

    Purpose

    Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoI

    Depositor

    Julia Vorholt

    Article

    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

    Inserts

    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    lacZYA
    

    Use

    Conjugative bacterial vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSW002-Pc-TorA(sp)

    Plasmid

    #111253

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorA signal peptide

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorA signal peptide
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pc

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-tdTEVDB_C-15A/G4C

    Plasmid

    #177205

    Purpose

    Permuted td intron with evolved initiation sequence for the expression of circular mRNA

    Depositor

    Stephen Fried

    Article

    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

    Insert

    Permuted GFP with evolved initiation sequence for circular mRNA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Permuted sfGFP after residue 52 & Changed Cyt…

    Promoter

    araBAD

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. T7 promoter + rpsL reporter plasmid

    Plasmid

    #156455

    Purpose

    A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    rpsL
    TcR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    tac and tet

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYC-Cas12g1-noncoding

    Plasmid

    #120880

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-Cas12g1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PliaI

    Plasmid

    #55177

    Purpose

    Bacitracin-inducible promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    liaI promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    liaI

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRE167

    Plasmid

    #118515

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRE166

    Plasmid

    #118514

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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