Skip to main content
Addgene
Showing: 1251 - 1300 of 15391 results

  13. pAGH51

    Plasmid
    #107255
    Purpose
    HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.
    Depositor
    Erin O'Shea
    Article
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    HU-FRB
    Use
    Synthetic Biology
    Tags
    FRB
    Expression
    Mutation
    Promoter
    native HU promoter
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107255-plasmid-map-sequence-id-207562

    Enlarge

  16. pU6_(GLuc)_INT[GFPApt]

    Plasmid
    #68428
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing the GFP aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68428-plasmid-map-sequence-id-120206

    Enlarge

  17. pU6_(GLuc)_INT[3xK-T]

    Plasmid
    #68429
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three kink-turns
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68429-plasmid-map-sequence-id-125557

    Enlarge

  18. XW83

    Plasmid
    #65841
    Purpose
    MosSCI-Pmig-13::QF::SL2::mCherry
    Depositor
    Kang Shen
    Article
    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi
    Insert
    OR74A quinic acid utilization activator (qa-1F)
    Use
    Tags
    mCherry
    Expression
    Worm
    Mutation
    The endogenous SphI, KpnI and SacI sites in QF ar…
    Promoter
    Available since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65841-plasmid-map-sequence-id-120571

    Enlarge

  23. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid
    #191212
    Purpose
    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay
    Depositor
    Mohan Babu
    Insert
    SARS-CoV-2 spike_WT (S SARS CoV-2)
    Use
    Tags
    HiBiT (high affinity small nanoluciferase fragmen…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191212-plasmid-map-sequence-id-437009

    Enlarge

  29. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Toru Hiratsuka
    Article
    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1-alpha promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    225958-plasmid-map-sequence-id-452543

    Enlarge

  36. pJL1-AcrA

    Plasmid
    #198214
    Purpose
    pJL1 plasmid encoding wildtype AcrA from C. jejuni containing 2 native internal glycosylation sites recognized by PglB and a C-terminal 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    AcrA
    Use
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198214-plasmid-map-sequence-id-393684

    Enlarge

Showing: 1251 - 1300 of 15391 results