Addgene

We narrowed to 2,387 results for: MEA;

Showing: 501 - 520 of 2387 results

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Galphai2-mTurquoise2

Plasmid

#69621

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai2-mTurquoise2

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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JAB3603

Plasmid

#190422

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_4XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_4XPhlFO::GFPint-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2493

Plasmid

#190375

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XLacIO-min35S::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XLacIO-min35S::GFPint-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2192

Plasmid

#190356

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XQacRO-min35S::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XQacRO-min35S::GFPint-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2191

Plasmid

#190355

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XLmrAO-min35S::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XLmrAO-min35S::GFPint-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.155_LV-Puro-T2A-mTagBFP2

Plasmid

#205553

Purpose

Express puromycin resistance marker and mTagBFP2

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Puro-T2A-mTagBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT430

Plasmid

#202291

Purpose

RPU integration plasmid (LPattB7)

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

J23101-RiboJ00-B0034-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCggRO-reporter

Plasmid

#124582

Purpose

Reporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)

Depositor

Matthias Heinemann

Article

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

CggR promoter element

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAJT254-litR

Plasmid

#202308

Purpose

litR repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

litR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT249-psrA

Plasmid

#202304

Purpose

psrA repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

psrA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE2-Hyg-omMx1

Plasmid

#52420

Purpose

Inducible expression (Tet-Off system) of the rainbow trout antiviral Mx1 protein

Depositor

Bertrand Collet

Article

Lester et al J Virol Methods. 2012 Jun;182(1-2):1-8.

Insert

Oncorhynchus mykiss interferon-induced GTP-binding protein Mx1 (mx1)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE2

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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At1R-P2A-mCherry

Plasmid

#112934

Purpose

Plasmid co-producing the AT1R receptor and a mCherry

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

AT1R

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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hisSUMO-ZFP57

Plasmid

#102716

Purpose

Express DNA binding domain of human Zinc finger protein 57 in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

Zinc finger protein 57 (ZNF57 Human)

Use

Tags

hisSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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Ga13_2-mTq2

Plasmid

#112933

Purpose

Human Galpha13 tagged with mTurquoise, inserted at residue 128

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

Galpha13

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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JAB2098

Plasmid

#190340

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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His6-TEV-Venus-pBluescript

Plasmid

#166756

Purpose

For purification of Venus fluorophore

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Venus

Use

Tags

6x Histidine tag, and TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pHO_pTEFmut7_CggR_R250A_ble

Plasmid

#124585

Purpose

Plasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoter

Depositor

Matthias Heinemann

Article

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

cggR_R250A (cggR Synthetic)

Use

Synthetic Biology

Tags

Expression

Mutation

changed arginine 250 to alanine

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJAG98-mTurquoise2-Nuf2

Plasmid

#80760

Purpose

Express Nuf2 N-terminally labeled with mTurquoise2

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

Nuf2 (NUF2 Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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G13 sensor (Ga13_2)

Plasmid

#112930

Purpose

A FRET sensor for G13 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

G13 sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GYB

Plasmid

#160927

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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