Addgene

We narrowed to 1,054 results for: proc.2

Showing: 501 - 520 of 1054 results

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pEYFP-Synectin

Plasmid

#35791

Depositor

Arie Horowitz

Article

Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

Insert

Synectin (Gipc1 Mouse)

Use

Tags

EYFP

Expression

Mammalian

Mutation

K111R

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-6p-hDcp2

Plasmid

#72216

Purpose

bacterial expression of GST-tagged human Dcp2

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

F146Y and E198K (please see depositor's comm…

Promoter

tac

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA4/HisMax-Synectin

Plasmid

#35943

Depositor

Arie Horowitz

Article

Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

Insert

Synectin (Gipc1 Mouse)

Use

Tags

6xHIS and Xpress

Expression

Mammalian

Mutation

K111R

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-hDcp2 E147Q, E148Q

Plasmid

#72215

Purpose

bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

E147Q, E148Q

Promoter

T7

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-24p3/LCN2-282-LUC

Plasmid

#46865

Purpose

murine 24p3 minimal promoter as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Tags

Expression

Mutation

Promoter

24p3 minimal promoter

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Use

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Promoter

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pCEP4_OCT4_T234A_S235A

Plasmid

#40630

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1_S234A_T235A (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

S234A_T235A

Promoter

EF1-alpha

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pCEP4_OCT4_T234E_S235E

Plasmid

#40631

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

T234E_S235E

Promoter

EF1-alpha

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pCNL-N1_PTS2

Plasmid

#65676

Purpose

Expresses PTS2-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-24p3/LCN2-145-LUC

Plasmid

#46866

Purpose

murine 24p3 minimal promoter as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Tags

Expression

Mutation

Promoter

24p3 promoter (inactive)

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pONL-N1_PTS2

Plasmid

#65677

Purpose

Expresses PTS2-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T-1_OCT4_T234A_S235A

Plasmid

#40634

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Use

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Mammalian

Mutation

T234A_S235A

Promoter

tac

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pYNL-N1_PTS2

Plasmid

#65675

Purpose

Expresses PTS2-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA6-TAF4-3xFLAG

Plasmid

#31901

Depositor

Robert Tjian

Article

Wright et al Proc Natl Acad Sci U S A. 2009 Jan 6;106

Insert

TAF4 (Taf4 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Marvin Wickens, Hugo Cesar Medina-Munoz

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Use

Tags

Expression

Yeast

Mutation

Promoter

PSEC63

Available since

Availability

Academic Institutions and Nonprofits only

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pLV-puro-N-3HA-SREBP2-C-Flag

Plasmid

#134287

Purpose

Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP2 (Srebf2 Mouse)

Use

Lentiviral

Tags

3x HA and Flag

Expression

Mammalian

Mutation

mouse SREBP2 precursor

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6-C-Flag-GK5_v2

Plasmid

#134282

Purpose

Expresses Flag-tagged GK5 (isoform 2) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINO_H11

Plasmid

#184277

Purpose

Expresses nanobody H11 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11

Use

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBP-parallel1-GK5_v2

Plasmid

#134288

Purpose

Expresses GK5 (isoform 2) in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Tags

MBP

Expression

Bacterial

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

T7

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCVD208

Plasmid

#159762

Purpose

Expression vector for wild-type Fc-fused ACE2(740)

Depositor

Jim Wells

Article

Glasgow et al Proc Natl Acad Sci U S A. 2020 Nov 10;11

Insert

ACE2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge