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EF1a_MYC_P2A_Hygro_Barcode

Plasmid

#120461

Purpose

Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC (MYC Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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DDX5

Plasmid

#155523

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX5 (DDX5 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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ACE2

Plasmid

#164219

Purpose

Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker

Depositor

Sriram Neelamegham

Article

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Mutation

silent mutation to remove internal EcoRI site (GA…

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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iBE4max YE1 NG CLYBL

Plasmid

#174570

Purpose

doxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-in

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

BE3

Expression

Mammalian

Promoter

tet ON

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO_FOXA1_#2

Plasmid

#70096

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

u6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pBK- Ma PylRS WT

Plasmid

#197573

Purpose

Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Insert

M. alvus PylRS WT

Expression

Bacterial

Promoter

GlnS (constitutive)

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF17_P2A_Hygro_Barcode

Plasmid

#120503

Purpose

Barcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF17 (KLF17 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJT302 Ec03 Lenti pEF-attB LSR GFP

Plasmid

#184945

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Ec03 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304-FOS-V5

Plasmid

#59140

Purpose

wild-type FOS with C-terminal V5 tag expressed from pLX vector

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

FOS (FOS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

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pAR17_StrepI_hFASh_H8_pET22b

Plasmid

#122846

Purpose

Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

Depositor

Martin Grininger

Article

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

human FASN/ human fatty acid synthase (FASN Human)

Tags

His8 tag and Twin-Strep tag

Expression

Bacterial

Mutation

wildtype

Promoter

T7 promoter

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJT303 Ec04 Lenti pEF-attB LSR GFP

Plasmid

#184946

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Ec04 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar1

Plasmid

#232738

Purpose

Expresses NADPH/NADP+ biosensor NAPstar1 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar1

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TAK1/F

Plasmid

#44161

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pJT304 Ec07 Lenti pEF-attB LSR GFP

Plasmid

#184947

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Ec07 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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EXT1- D164A-pLJC2-3XFLAG

Plasmid

#215828

Purpose

Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 Mutant (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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CLK1

Plasmid

#155458

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CLK1 (CLK1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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TARDBP

Plasmid

#156073

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TARDBP (TARDBP Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/8]

Plasmid

#199429

Purpose

Mammalian expression of GFP scFv. Derived from hybridoma N86/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingGFP (Aequorea victoria)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa

Plasmid

#233034

Purpose

To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d and mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_ATG101

Plasmid

#223796

Purpose

Expression of recombinant protein for purification (co-expression with ATG13)

Depositor

James Hurley, Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG101 (ATG101 Human)

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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