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LXSN-Cx43-S279/282D

Plasmid

#60715

Purpose

retroviral expression of rat Cx43 S279/282D mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

S279/282D

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
TFORF0814

Plasmid

#141716

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

LHX1 (LHX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-HA-Mettl3

Plasmid

#196262

Purpose

In vitro transcription/translation of HA-tagged wild-type Mettl3

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Mettl3 (Mettl3 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

CMV IE94

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pME mKate2-dynactin1-811

Plasmid

#105971

Purpose

entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

dynactin1-811 (DCTN1 Human)

Tags

mKate2

Expression

Bacterial

Mutation

contains only amino acids 1-811

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH

Plasmid

#203841

Purpose

non-functional Kir2.1(mutant) expression as a control for the silencing of neurons with Kir2.1 and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Has Service

AAV2

Insert

Kir2.1 (Kcnj2 Mouse)

Use

AAV

Tags

2A-mScarlet-KASH

Mutation

G144A, Y145A, G146A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3444

Plasmid

#144920

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

KCNIP2 (KCNIP2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Mouse aB crystallin 250bp promoter/AcGFP

Plasmid

#98102

Purpose

Mouse aB crystallin 250bp promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha B-crystallin promoter 250 bp fragment, Mus musculus (Cryab Mouse)

Use

Gfp expressing

Tags

GFP

Promoter

Alpha B-crystallin promoter 250 bp fragment (-259…

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF0903

Plasmid

#141752

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SPDEF (SPDEF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_LN-HA-AGO2-5xA

Plasmid

#92010

Purpose

Expresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSLIK mCh-V5-NES-EGFP-ERBB2 NLS zeo

Plasmid

#192338

Purpose

Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-ERBB2 NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-ERBB2 NLS (ERBB2 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC35B3

Plasmid

#132087

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35B3 (SLC35B3 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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mVenus-proα2(I)

Plasmid

#119838

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with mVenus between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

mVenus

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag

Promoter

CMV

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Mouse aB crystallin 800bp promoter/AcGFP

Plasmid

#98103

Purpose

Mouse aB crystallin 800bp promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha B-crystallin promoter 800 bp fragment, Mus musculus (Cryab Mouse)

Use

Gfp expressing

Tags

GFP

Promoter

Alpha B-crystallin promoter 800 bp fragment (-833…

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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tdEos-Actin-C-18

Plasmid

#57571

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Actin (ACTB Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1+_LN-HA-AGO2-5xE

Plasmid

#92011

Purpose

Expresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBS L30 Ruby3-Rab7A

Plasmid

#135651

Purpose

Rab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab7A (RAB7A Human)

Tags

Ruby3

Expression

Mammalian

Promoter

L30

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-cAICDA-BE3

Plasmid

#113422

Purpose

Expresses cAICDA-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

cAICDA-BE3 (aicda Ictalurus punctatus (channel catfish))

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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INSR gRNA (BRDN0001145804)

Plasmid

#75506

Purpose

3rd generation lentiviral gRNA plasmid targeting human INSR

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

INSR (INSR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMP108 Nanog-Venus-2a-mCherry

Plasmid

#159743

Purpose

Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.

Depositor

Sergi Regot

Article

Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d

Insert

Nanog homeobox (Nanog Mouse)

Use

Mouse Targeting

Tags

Venus-2a-mCherry

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMCh2007_pLenti_Syn_mChe-cdc42E7

Plasmid

#118620

Purpose

expression of mChe-tagged cdc42E7 under pSyn promoter for primary neurons

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

changed cagtctg to ggcataa (667 - 673 nt in 3…

Promoter

synapsin I (rat)

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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