We narrowed to 155,828 results for: ins;
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Plasmid#158060PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyExpressionBacterialPromoteracpPAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+)IRES GFP
Plasmid#51406PurposeExpresses GFP from internal ribosome entry sequence, cloning componentDepositorInsertIRES GFP
ExpressionMammalianPromoterCMVAvailable SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-N1
Plasmid#60561PurposeExpresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteinsDepositorInsertmTurquoise2
ExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G
Plasmid#167935PurposeInducible CRISPRi expressionDepositorInsertKRAB-dCas9-DHFR
UseLentiviralPromotertetONAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-144:CTCF-mEGFP
Plasmid#168799PurposeHomology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCFDepositorInsertCTCF (CTCF Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceJune 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-166:CDH1-mEGFP
Plasmid#193921PurposeHomology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1DepositorInsertcadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
UseAAV and CRISPR; Donor templateTagslinker-mEGFPMutationhomology arms contain point mutations to disrupt …Available SinceDec. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
SpyCatcher002-oDi
Plasmid#124661PurposeExpresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coilDepositorInsertSpyCatcher002-oDi
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Lifeact-EGFP-2XNLS
Plasmid#58467PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoterDepositorInsertsLifeact
2X NLS with 2X stop codons
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pFA6a-link-ymNeongreen-URA3
Plasmid#168059PurposeReplaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymNeonGreen
TagsymNeonGreenExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
VPS13A^mCherry
Plasmid#118758PurposeVPS13A internally tagged with mCherry at residue 1372.DepositorAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEff
Plasmid#97423PurposeTransient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI-URA3
Plasmid#168055PurposeReplaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymScarletI
TagsymScarletIExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ytdTomato-URA3
Plasmid#168054PurposeReplaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertytdTomato
TagsytdTomatoExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymYPET-URA3
Plasmid#168056PurposeReplaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymYPET
TagsymYPETExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIMcpf1
Plasmid#153034PurposeFor CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.DepositorInsertAscpf1
ExpressionBacterialAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymTq2-URA3
Plasmid#168057PurposeReplaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymTurquoise2
TagsymTurquoise2ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only